• Login
    View Item 
    •   ResearchSpace Home
    • College of Health Sciences
    • School of Laboratory Medicine & Medical Sciences
    • Microbiology and Infection Control
    • Masters Degrees (Microbiology and Infection Control)
    • View Item
    •   ResearchSpace Home
    • College of Health Sciences
    • School of Laboratory Medicine & Medical Sciences
    • Microbiology and Infection Control
    • Masters Degrees (Microbiology and Infection Control)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Neutrophil cytoplasmic antibodies : their clinical associations and an improved method for their detection.

    Thumbnail
    View/Open
    Duursma_June_1993.pdf (2.497Mb)
    Date
    1993
    Author
    Duursma, June.
    Metadata
    Show full item record
    Abstract
    The test for antineutrophil cytoplasmic antibodies (ANCA) was introduced into the author's laboratory in 1987. An improved indirect immunofluorescent method was developed, using a system which allows 16 instead of one serum sample to be screened on each microscope slide. The known disease associations of ANCA that have been explored include systemic vasculitis, renal limited vasculitis, chronic inflammatory bowel disease and HIV disease. In general the findings are similar to those which are emerging from other centres and confirm the value not only of the positivity but also the relevance of the intracellular disposition of the neutrophil cytoplasmic fluorescence in diagnosis. In this study 85% of patients with Wegener's granulomatosis were found to have C-ANCA. C, P and X-ANCA staining patterns were found in 57% of patients with ulcerative colitis. Forty one per cent of patients with symptomatic HIV have ANCA. Certain histological features such as neutrophil and vascular damage in invasive amoebiasis, and the established lytic effect of amoebae on neutrophils prompted the investigation of the possibility that ANCA may be generated in this disease. Seventy eight amoebiasis sera were screened and 98,70/0 gave a positive ANCA test with a pattern of fluorescence resembling that found in Wegener's granulomatosis. An ELISA test for specificity confirmed that, as in Wegener's granulomatosis, this amoebiasis-associated ANCA had proteinase 3 specificity. Of practical clinical importance is the fact that both HIV and amoebiasis are associated with a high level of ANCA positivity. These findings will need to be considered when ANCA tests are used in clinical decision making in an area where HIV disease and amoebiasis are endemic. A large number of normal volunteer blood donors have been tested and the false positivity rate of 0,5% confirms the specificity of the test.
    URI
    http://hdl.handle.net/10413/7360
    Collections
    • Masters Degrees (Microbiology and Infection Control) [24]

    DSpace software copyright © 2002-2013  Duraspace
    Contact Us | Send Feedback
    Theme by 
    @mire NV
     

     

    Browse

    All of ResearchSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsAdvisorsTypeThis CollectionBy Issue DateAuthorsTitlesSubjectsAdvisorsType

    My Account

    LoginRegister

    DSpace software copyright © 2002-2013  Duraspace
    Contact Us | Send Feedback
    Theme by 
    @mire NV