Repository logo

Doctoral Degrees (Electrical Engineering)

Permanent URI for this collectionhttps://hdl.handle.net/10413/6855

Browse

Browsing Doctoral Degrees (Electrical Engineering) by Author "Chetty, Leon."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    DC coronation electroporation.
    (2015) Chetty, Nevendra Krishniah.; Davidson, Innocent Ewean.; Chetty, Leon.; Govender, T.; Ijumba, Nelson Mutatina.
    Cells are surrounded by a semi-permeable bilayer lipid membrane that acts as a barrier against the entry of foreign molecules. In the fields of molecular biology, biotechnology, and medicine, the ability to breach the cell membrane and introduce molecules into cells for therapeutic purposes is often necessary. Molecules, which are considered foreign to the cell like drugs and extraneous genetic materials, are administered to cells for numerous applications including the treatment and prevention of diseases. There are many accepted methods of facilitating the delivery of molecules to cells. Of all these methods, one important and well-established physical method is electroporation which has been utilised for decades. Electroporation is a widely adopted procedure for the temporary permeabilization of cell membranes due to the application of short electrical pulses. It is a phenomenon resulting from the effects of pulsed electric fields, which induces biochemical and physiological changes to a cell membrane. As a result, some of the molecules that are ordinarily unable to pass through the membrane are thereafter able to gain access to the cell interior via pores that are formed in the membrane. Even though electroporation is fairly safe, there are some drawbacks associated with this method. The traditional method of electroporation requires direct contact of high voltage electrodes and fairly high currents are involved. As a result, the procedure can cause pain, muscle spasms, discomfort, burning and cell and tissue damage. Alternative methods of molecular delivery are therefore being researched, especially non-contact methods such as the use of high voltage plasma and high voltage corona discharge. Successful cell permeabilization with corona discharge ions and plasma has been previously demonstrated. These methods offer the advantage of contact-free treatment with low associated current. In this thesis, the research investigates the delivery of tracer molecules, SYTOX Green, into HeLa cells and the consequential cell destruction by the phenomenon of corona discharge. A high voltage DC, multipoint-to-plane atmospheric-air corona discharge apparatus was designed and constructed to investigate the conditions as well as the characteristics of the corona discharge current pulses that resulted in an acceptable balance between high cell permeabilization and low cell destruction. Firstly, the salient variables that affect molecular delivery and cell destruction were established. Secondly, the variables were optimized to allow for reliable molecular delivery to cells with acceptable levels of cell destruction. Thirdly, the nature and variation of the corona discharge current pulses and its effect on molecular delivery and cell destruction were investigated. Finally, a new method of assessing cell destruction, which combined the measurements of cell viability and cell lysis were used. The variables that were identified, over the course of many experiments, were exposure time to corona discharge, incubation time with SYTOX Green, volume of liquid during exposure, and inter-electrode distance. Further experiments show that when the variables of the experiment are set at optimal values, cell permeabilization is reliable with minimal damage to cells. Once these conditions were obtained and optimised, the effect of different applied voltages on the level of cell permeabilization and the short-term destructive effects on cells were investigated. The general trend is an increase in fluorescence and therefore, molecular delivery, with an increase in applied voltage. Cell destruction also tends to increase with increasing applied voltage. The characteristics of the corona current pulses that were analyzed include amplitudes, repetition rates, widths, and rise-times. The characteristic frequencies of single pulses, obtained from the application of a discrete fast Fourier transform, were also analyzed. For the corona-generating device constructed and the voltages tested, it was found that the only characteristic that varies appreciably with voltage is the pulse repetition rate. A higher pulse repetition rate relates to a greater number of pulses per unit time and therefore, a greater exposure of the cells to the applied electric field. This would, therefore, translate to a higher extent of molecular delivery and a higher accompanying level of cell destruction. This study shows that permeabilization of HeLa cells due to corona discharge can be reliably achieved and the results provide a greater understanding of cell permeabilization due to the influence of corona discharge. It therefore forms an important basis for future research on practical applications that would promote the establishment and acceptance of corona discharge as a procedure for molecular delivery to cells.
  • Thumbnail Image
    Item
    Design synthesis of LCC HVDC control systems.
    (2011) Chetty, Leon.; Ijumba, Nelson Mutatina.
    From the early days of HVDC system applications, the importance of mathematical modelling of the dynamics of Line Commutated Converter (LCC) HVDC systems has been appreciated. There are essentially two methodologies used to develop mathematical models of dynamic systems. One methodology is to define the properties of the system by the “laws of nature” and other well-established relationships. Basic techniques of this methodology involve describing the system’s processes using differential equations. This methodology is called “Deductive Modelling”. The other methodology used to derive mathematical models of a dynamic system is based on experimentation. Input and output signals from the original system are recorded to infer a mathematical model of the system. This methodology is known as “Inductive Modelling”. A review of the current state of the art of modelling LCC HVDC systems indicates that majority of the techniques utilized to develop mathematical models of LCC HVDC systems have used the “Deductive Modelling” approach. This methodology requires accurate knowledge of the ac systems and the dc system and involves complicated mathematics. In practice, it is nearly impossible to obtain accurate knowledge of the ac systems connected to LCC HVDC systems. The main aim of this thesis is to present an “Inductive Modelling” methodology to calculate the plant transfer functions of LCC HVDC systems. Due to the uncertain nature of the effective short circuit ratio of rectifier and inverter converter stations, generic ranges of parametric uncertainties of the developed plant transfer functions were determined. Based on the determined range of HVDC plant parametric uncertainty, Quantitative Feedback Theory (QFT) methodology was used to design the parameters of the LCC HVDC control system. The stability of the start-up and step responses for varying ac system conditions validated the “Inductive Modelling” technique and the QFT design methodology. The thesis presents the following, which are considered to be scientific advancements and contributions to the body of knowledge: · Novel LCC HVDC Step Response (HSR) equations were developed using an “Inductive Modeling” technique. · The range of parametric variations of the LCC HSR equations were determined for various rectifier and inverter ac system effective short circuit ratios. · The LCC HSR equations were used to develop the LCC HVDC plant transfer functions for various rectifier and inverter effective short circuit ratios. · The LCC HVDC plant transfer functions were utilized to design an LCC HVDC control system for varying ac system conditions using Quantitative Feedback Theory (QFT) methodology. The main contributions of this thesis relate to LCC HVDC systems. This thesis does not attempt to advance control theory however this thesis does apply existing classical control theory to LCC HVDC control systems. Index Terms: Line Commutated Converter, HVDC, inductive modelling, power system, transient analysis.