The evaluation of the health status of Clarias gariepinus from the Inanda and Nagle dams in KwaZulu-Natal, South Africa.

Abstract

The uMgeni River is one of the most polluted freshwater ecosystems in KwaZulu-Natal. This river is home to about 48 fish species. The study aims to determine the effects of water quality on the health of Clarias gariepinus from the Nagle and Inanda dams. Water variables were measured in situ using a YSI meter. Water and sediment samples were collected at three sites in each dam. Fish were collected and euthanized by severing the spinal cord. Different fish biometrics were measured. Fish tissues were preserved based on the analysis to be carried out. Neutral to alkaline water pH was recorded at both dams. Although the Inanda Dam exhibited higher total nitrogen concentration, both dams were mesotrophic, whereas the phosphate concentration at the Inanda Dam was eutrophic. Generally, the Nagle Dam showed good quality water compared to the Inanda Dam. The Inanda Dam fish population showed a relatively higher prevalence of alterations in the gills and liver than fish from the Nagle Dam. The degree of alterations showed some variability within each population, however, there was no significant difference (p>0.05) between the two populations. Both populations exhibited organ indexes less than 20, hence, moderate alterations. Fish ovaries and testis showed slight alterations at both dams. Acetylcholinesterase (AChE) brain activity recorded no significant difference in between the dams (p>0.05). Male populations recorded no significant difference in vitellogenin (VTG) induction between the dams (p>0.05). Despite, AChE activity and VTG induction showing no significant difference between the two populations, there was a great variability within each population. The lowest AChE activity as well as the highest VTG level were observed in the Inanda Dam fish populations. Both histopathologic and biochemical biomarkers are signs of increased pollution effect at the Inanda Dam. Nevertheless, it is evident that the uMgeni River system which is supposed to be providing sanctuary to aquatic biota is becoming deteriorated. These findings provide baseline data or a point of reference for future studies as it is the first of its kind in this river system.