Genetic variation and associations among adaptive traits in a recombinant maize inbred line population.

Abstract

Maize production in Africa is constrained by abiotic and biotic stresses. Breeders need to have information on the nature of combining ability of parents, their traits and performance in hybrid combination. This requires careful determination of genetic variability of parents, and studying associations between grain yield and adaptive traits to breed superior cultivars which are better able to withstand such stresses. Therefore, this study was aimed at selecting parental testers with best combining ability in hybrid combination with recombinant inbred lines (RILs); and studying the correlation between grain yield and its components in eastern and western South Africa. It was also aimed at determining genetic variation and associations among adaptive traits in hybrids involving RILs. The final objectives of the study were to determine cultivar superiority of testcrosses involving RILs, and to select the best cultivars within and across four different environments. The 42 RILs were crossed to 9 Zimbabwean tropical testers resulting in 1009 hybrids with sufficient seed for planting in trials. From these a sample of 87 hybrids with adequate seed were selected and planted at four sites for combining ability analysis. The hybrids were evaluated at four sites in two regions; western region (Potchefstroom research station) and eastern region (Cedara, Ukulinga and Dundee research stations), during 2011/12 season. The experiments were laid out as augmented alpha lattice design. Trials were managed in accordance with production culture for each region. All quantitative data was subjected to GenStat and SAS statistical softwares. The results from combining ability study indicated that the line general combining ability (GCA) effects played a non-significant role (p > 0.05) in determining grain yield, grain moisture and anthesis date, while they were significant (p ≤ 0.05) for the other traits such as ear prolificacy. The tester main effects were significant for all the traits except ear prolificacy and plant height. Results also revealed that all the traits were controlled by both additive and non-additive genes, where additive gene action had the most contribution to the traits. The non-additive gene action played a minor role suggesting the total GCA effects attributed to both lines and testers predominantly higher over the specific combining ability (SCA) for all traits. In general the additive effects were preponderant over the non-additive gene effects. One cross (L114 x T12) had a significant and positive SCA effect for grain yield. The correlation between grain yield and secondary traits (number of ears per plant, grain moisture content, ear height, plant height, ear position and anthesis date) suggested that indirect selection can be employed to enhance grain yield by breeding for these particular adaptive traits. Path analysis showed that plant height had the highest direct and indirect effect on grain yield indicating its importance among other secondary traits for grain yield enhancement. Phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV) for all the studied traits across all the four environments. All the traits displayed high heritability at Potchefstroom except anthesis date which was highly heritable at Ukulinga. Cedara was the second best site for heritability of all the traits except for the number of ears per plant. The genetic advance for grain yield was the highest at Cedara followed by Potchefstroom, Dundee and Ukulinga. The hybrids exhibited different patterns of variation and distribution for all the traits. This indicated that selection strategies to exploit GCA should be emphasised. Association studies among grain yield and secondary traits such as ear length, number of ears per plant, plant height, anthesis date, silking date and ear leaf area revealed that there were significant phenotypic correlations between grain yield and secondary traits, and among the secondary traits. Ear length had the highest direct effect on grain yield at Ukulinga; number of ears per plant had the highest direct effect on grain yield at Cedara and Potchefstroom; whereas plant height had the highest direct effect on grain yield at Dundee. Grain yield was least affected by indirect factors at all the sites except Ukulinga, where anthesis date had the highest indirect effect on grain yield through silking date followed by plant height through leaf area. The study reveals that there is significant variation among the hybrids for mean performance, indicating that there is opportunity for selection. Overall the findings suggest that direct selection would be appropriate to enhance grain yield. Path analysis revealed that plant height had the highest direct and indirect effects on grain yield, indicating that plant height can be further exploited as the main trait in future breeding programmes for grain yield increment. Hybrid 10MAK10-1/N3 was the best hybrid at Ukulinga in terms of grain yield, relative yield and economic traits. Whereas hybrid T17/L83 was the best hybrid at Cedara in terms of grain yield and relative yield; however, T11/L102 was selected as the most elite hybrid with respect to grain yield, relative yield and economic traits. Hybrid T3/L48 was identified as thebest hybrid at Dundee with respect to grain yield, relative yield and prolificacy. At Potchefstroom the standard check PAN6611 was identified as the best hybrid in terms of grain yield and relative yield followed by developmental hybrid T1/L28; however, developmental hybrid T1/L28 was the best in terms of earliness, prolificacy and ear aspect. Stability coefficients and cultivar superiority index across the sites revealed that four developmental hybrids were identified as best hybrids and they performed better than the standard check. These hybrids will be recommended for further testing in advanced trials. With respect to cultivar superiority, the desired hybrids are required to combine high grain yield with economic and adaptive traits such as high ear prolificacy, low grain moisture, and low ear aspect score (desired) for them to adapt to production environments in South Africa. There was significant variation among the top 25 yielding hybrids. At least 5 hybrids combined high grain yield with the desired complimentary adaptive traits such as quick moisture dry down, prolificacy and ear aspect. The results showed that there is variation in the performance of high yielding genotypes within all the sites, and that agronomically superior cultivars can be identified. The study shows that there is significant variation among the RILs since they interacted differently with the 9 tropical testers. Even among the top 25 selections of RILs in each environment there was still variation for combinations of the desired traits. Significant associations among grain yield and other economic and adaptive traits were observed with implications for breeding strategy. Above all the significant variation gives large score for future breeding of new unique lines.