Repository logo
 

The effects of naringin on glucose tolerance and ketoacidosis in type 1 diabetes.

dc.contributor.advisorOwira, Peter Mark Oroma.
dc.contributor.authorNeondo, Murunga Alfred.
dc.date.accessioned2016-05-19T07:52:52Z
dc.date.available2016-05-19T07:52:52Z
dc.date.created2014
dc.descriptionM. Pharm. University of KwaZulu-Natal, Durban 2014.en_US
dc.description.abstractThe effects of naringin (4’,5,7-trihydroxy flavonone-7-rhamnoglucoside), a flavonoid isolated from the grapefruit and other citrus fruit species were investigated on diabetic ketoacidosis (DKA) in a type 1 diabetic rat model. DKA is an acute life threatening complication of diabetes mellitus. Male Sprague-Dawley rats (225-250g) were divided into 5 groups (n=7). Group 1 (control) was treated daily with 1.0 ml/kg distilled water while group 2 was treated with 50 mg/kg of naringin, via gastric gavage respectively. Diabetes was induced in groups 3, 4 and 5 by a single intraperitoneal injection of 60 mg/kg streptozotocin in 0.1 M citrate buffer and was confirmed after 48 hours. Group 3 was further treated with subcutaneous insulin (4 IU/kg) twice daily respectively. Blood samples for analysis were collected by cardiac puncture. The animals were handled humanely according to the guidelines of the Animal Ethics Committee, University of KwaZulu Natal, number 106/13/Animal. The untreated diabetic rats (group 5) showed significant (p<0.0001) hyperglycemia, polydipsia, polyuria, weight loss, impaired glucose tolerance, low fasting plasma insulin (FPI) and low glycogen levels compared to the normal control. They also showed significantly (p<0.0001) elevated acetoacetate (AcAc), β-hydroxybutyrate (3HB), total ketone body (TKB), anion gap (AG) and potassium (p<0.05) levels compared to normal control. Furthermore, significant (p<0.01) reductions blood pH, sodium, chloride and bicarbonate (p<0.0001) levels were recorded compared to the normal control. Treatment of the diabetic groups with naringin did not improve fasting blood glucose and serum electrolyte levels but significantly improved weight loss (p<0.0001), water consumption (p<0.0001), hepatic glycogen level (p<0.05), 3HB (p<0.05), AcAc (p<0.05), TKB (p<0.01), bicarbonate (p<0.01), blood pH (p<0.01) and AG (p<0.05) compared to the diabetic control group. The results in this study therefore suggest that naringin reverses ketoacidosis but does not improve glucose tolerance in a diabetes type 1 rat model.en_US
dc.identifier.urihttp://hdl.handle.net/10413/13008
dc.language.isoen_ZAen_US
dc.subjectGrapefruit.en_US
dc.subjectDiabetes.en_US
dc.subjectGlucosides.en_US
dc.subjectGlucose tolerance tests.en_US
dc.subjectTheses--Pharmacy and pharmocology.en_US
dc.subjectNaringin.en_US
dc.titleThe effects of naringin on glucose tolerance and ketoacidosis in type 1 diabetes.en_US
dc.typeThesisen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Neondo_Murunga_Alfred_2014.pdf
Size:
1.45 MB
Format:
Adobe Portable Document Format
Description:
Theses

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.64 KB
Format:
Item-specific license agreed upon to submission
Description: