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    Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction.

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    Journal article (830.5Kb)
    Date
    2011
    Author
    Hong, H. A.
    Loubser, A. S.
    de Assis Rosa, Debra.
    Naranbhai, Vivek.
    Carr, William Henry.
    Paximadis, Maria.
    Lewis, David A.
    Tiemessen, Caroline T.
    Gray, Clive M.
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    Abstract
    The effector function of natural killer (NK) cells is modulated by surface expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that interact with human leukocyte antigen (HLA) class I ligands. We describe the use of real-time polymerase chain reaction (PCR) assays that allow easy and quick detection of 16 KIR genes and the presence/absence of KIR-ligands based on allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. These methods overcome the tedious and expensive nature of conventional KIR genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing (SBT). Using these two cost-effective assays, we measured the frequencies of KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited in South Africa.
    URI
    http://dx.doi.org/10.1111/j.1399-0039.2011.01749.
    http://hdl.handle.net/10413/7998
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    • Research papers (Caprisa) [447]

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