Biochemistry
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Recombinant cathepsin L-like cysteine proteases for species-specific diagnosis of animal African trypanosomiasis.
(2019)African trypanosomiasis is a disease caused by protozoan parasites i.e. Trypanosoma spp. in livestock and humans and affects 37 sub-Saharan countries. Animal African trypanosomiasis (AAT) is known as nagana and African ... -
Molecular cloning, recombinant expression and characterisation of serine and cysteine protease inhibitors from Trichinella zimbabwensis.
(2019)Trichinellosis is a disease caused by parasitic helminths of the genus Trichinella. Infection occurs by ingestion of meat contaminated with infective Trichinella larvae. Cases of Trichinella zimbabwensis, a non-encapsulating ... -
The stabilisation of the cysteine protease of Carica papaya (papain) and the catalytic domain of the Cathepsin L-like cysteine protease of Trypanosoma congolense (TcoCATL)
(2019)Proteases play an intricate role in the numerous functions of a living organism. Proteases are responsible for the cleavage of proteins into smaller fragments by catalysing the hydrolysis of peptide bonds. The class of ... -
Transferrin receptor-mediated gene delivery using functionalised gold nanoparticles.
(2018)Gene therapy strategies have shown their potential in treating numerous central nervous system (CNS) disorders, including highly aggressive brain cancers. Gold nanoparticles (AuNPs) are popular vectors for gene delivery, ... -
Expression and evaluation of a 297-amino acid fragment of the blood stage protein PfC0760c from Plasmodium falciparum.
(2019)Abstract available in pdf. -
Evaluating the effect of nitric oxide on myoblast proliferation, migration and differentiation.
(2018)Wound healing is the process of restoring tissue integrity in response to injury. This process involves four major phases namely; haemostasis, inflammation, regeneration and remodelling. These phases are regulated by ... -
Mechanisms behind the anti-diabetic effects of caffeine in a Type 2 diabetes model of rats.
(2018)Caffeine has been used for many years and is one of the most extensively consumed active food ingredient throughout the world. Caffeine has been broadly studied in a variety of areas regarding human health and performance. ... -
Recombinant expression and initial characterisation of two Plasmodium falciparum copper binding proteins: Cox11 and Cox19.
(2018)Emerging drug resistance hinders the efforts to control malaria and so novel antimalarial drugs are required. Copper is essential for the survival of plasmodial parasites but the proteins involved in copper homeostasis are ... -
Anti-c-myc cholesterol-based lipoplexes: development, characterisation and evaluation as Onconanotherapeutic agents in vitro.
(2018)Strategies aimed at inhibiting the expression of the c-myc oncogene could provide the basis for alternative cancer treatment. In this regard, silencing c-myc expression using small interfering RNA (siRNA) is an attractive ... -
Recombinant expression, purification, analysis and immunization of mice with plasmodium yoelii glyceraldehyde-3-phosphate dehydrogenase (rPyGAPDH) and lactate dehydrogenase (rPyLDH) and evaluation of protection against P. berghei challenge.
(2019)The principal focus in malaria research is the development of an effective malaria vaccine which will help to prevent death and eliminate the disease. This study investigated two plasmodial glycolytic enzymes as possible ... -
An investigation into the analytical, cytotoxicity and immunotoxicity of mycotoxins found in commercially available pelleted pet foods in Durban, South Africa.
(2018)Introduction: Dry pelleted dog food in the South African market is available via supermarket, pet stores (standard brands - SB) and veterinary channels (premium brands-PB). Similarly, cat food were viewed in two market ... -
Developing in vitro multi co-culture models and analysis tools for muscle regeneration.
(2019)Skeletal muscle regeneration represents a complex process mediated by non-myogenic cell types. These cells, such as macrophages and fibroblasts, display a range of interactions with muscle stem cells (myoblasts) during ... -
Evaluation of the anti-diabetic potentials of some African medicinal plants: a multimode study.
(2018)The present study investigated the effects of five African medicinal plants (Alstonia boonei, Acalypha wilkesiana, Boerhaavia diffusa, Bridelia ferruginea, and Crassocephalum rubens) for their antioxidative and antidiabetic ... -
The impact of aromatic ring count on the bioavailability of chemical compounds.
(2019)Aromatic rings are a negative contributing factor in the bioavailability of chemical compounds in drug discovery. Due to the inevitable effect the aromatic rings possess on the bioavailability of chemical compounds, methods ... -
Development of a novel biochemical assay for the identification of promiscuous compounds.
(2019)Promiscuous compounds, specifically aggregate-based inhibitors that result in false positives in biochemical activity assays present a serious and increasing interference to early-stage drug discovery processes. Although ... -
The evaluation of novel metal -based compounds as potential BACE1 ligands.
(2018)The proposed primary cause of Alzheimer’s disease (AD) is the cleavage of the Amyloid-β precursor protein by the β- Amyloid cleavage enzyme 1 (BACE1). As such it has been proposed that inhibiting this enzyme would help ... -
Antidiabetic and toxicological properties of some African medicinal plants used in the treatment of diabetes and its complications.
(2018)This study investigated the antioxidant, antidiabetic and toxicity properties of antidiabetic medicinal plants comprising of Vernonia amygdalina, Cola nitida, Raffia palm (Raphia hookeri) wine, Phaseolus lunatus, Dacryodes ...
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