Testing the utility of DNA barcoding in South African Hemiptera: using eThekwini species as a case study.
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Date
2017
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Abstract
The eThekwini municipal region and surrounding areas (Durban, Kwazulu-Natal, South Africa) are situated within the globally important Maputaland-Pondoland-Albany biodiversity hotspot. The biodiversity present in this region is under significant pressure from urbanization and climate change. This highlights the need to provide tools that can assist in the discovery and identification of species at an accelerated pace, to create biodiversity inventories which can be used for appropriate conservation planning. The creation of species inventories is a difficult task, more especially for hyper-diverse groups such as terrestrial arthropods. These groups can be morphologically cryptic or difficult to identify using traditional morphology-based taxonomy. Therefore, molecular-based methods of species identification have been proposed to assist in traditional taxonomy. DNA barcoding has been suggested as a mechanism which enables biologists to “label” or “tag” species, using nucleotide variations in short sequences known as DNA barcodes. This study investigates the utility of DNA barcoding and the use of the mitochondrial cytochrome oxidase c subunit 1 (COI) marker to identify species of Hemiptera efficiently and accurately. This study presents a preliminary DNA barcode reference library for Hemiptera collected from 18 different localities within and around the eThekwini municipal region. To test the success of DNA barcoding and the COI marker, matches between morphospecies and barcode clusters (BINs) were analyzed and the presence of the DNA barcode gap in the data was examined. The DNA barcode gap is the gap between the intraspecific and interspecific genetic distances, the lack of the DNA barcode gap suggests that taxa cannot be reliably sorted into species based on the genetic data. Analyses revealed that DNA barcoding using the COI marker is a successful method of identifying Hemiptera species in this study. Thereafter, a case study was selected within the Buffelsdraai Landfill Site Community Reforestation Project, to test whether DNA barcoding could be used to assess the potential of Hemiptera as an indicator of ecological restoration success. The Hemiptera species composition and assembly were assessed by analyzing multiple diversity indices, ordination, UPGMA cluster analysis and phylogenetic analysis. Hemiptera was seen to be sensitive to changes in an ecosystem which make this order an effective environmental and biological indicator. With the help of DNA barcoding, specific families of Hemiptera were identified as habitat-specific and good biological indicators for future studies of ecological restoration and reforestation.
Description
Master of Science in Genetics. University of KwaZulu-Natal, Durban 2017.
Keywords
Theses--Genetics., Hemiptera.