The effect of 6-Benzyladenine on adventitious shoot formation by Lycopersicon species in vitro.
Loading...
Date
1993
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Lycopersicon esculentum Mill. cv. Rodade was developed in South Africa for the
fresh produce market. This cultivar is also of major importance for South African
tomato breeding programmes because of its resistance to bacterial wilt. In this
study, aspects of the effects of 6-benzyladenine on adventitious shoot formation by
both L. esculentum cv. Rodade and Lycopersicon peruvianum Mill. were studied in
vitro. These included the regeneration of adventitious shoots, the effects of
different incubation times, the uptake and metabolism of BA and the effect of auxin
on the metabolism of BA in both leaf and callus tissue of the two species.
Adventitious buds could be regenerated on all tissue types except for callus tissue
of L. esculentum. A stepwise increase in the percentage shoots produced was
observed indicating a period of induction wherein incubation on a medium
containing BA is beneficial to the production of shoots. Leaf tissue was more
responsive to BA treatments than callus tissue of both species. The main route of
BA metabolism in both species is from BA to [9R]BA and [9R-MP]BA. Callus
tissue of L. esculentum cv. Rodade however converted BA to the 3- and 9-
glucosides of BA rather than to metabolically active forms of the cytokinin. The
auxin, indole-3-acetic acid, played a definite role in the conversion of BA to
[3G]BA and [9G]BA in leaf tissue of the tomato cultivar tested, but had no effect
in callus tissue of this species.
Description
Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1993.
Keywords
Lycopersicon spp., Roots (Botany), Tomatoes--Genetics., Tomatoes--Biotechnology., Tomato breeding., Theses--Botany.