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Masters Degrees (Horticultural Science)

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Browsing Masters Degrees (Horticultural Science) by Subject "Avocado--Postharvest losses--Prevention."

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    Effect of systemic resistance inducers applied pre- and postharvest for the development of a potential control of colletotrichum Gloeosporioides on Persea Americana (Mill.) CV 'Fuerte'.
    (2012) Bosse, Ronelle Joy.; Bertling, Isa.
    Avocados are one of the major food sources in tropical and subtropical regions and are an important horticultural crop in South Africa. Avocados are exported over long distances and may have storage times of up to 30 or more days at temperatures of about 5.5oC. This procedure increases the risk of poor fruit quality, including physiological disorders, early softening and postharvest disease incidence. A major component of the postharvest diseases is Anthracnose caused by Colletotrichum gloeosporioides. Anthracnose infects unripe fruit and once infected, the fungus remains dormant in the fruit until ripening begins. This leads to a problem for producers and packers, as the presence of the disease cannot be detected on the pack line, and fruit is not removed. Anthracnose control is normally done through pre-harvest treatment with copper-based fungicides. While effective such treatment needs to be repeated frequently, resulting in copper residues on the avocados. The study was conducted to investigate the effects of phosphoric acid and potassium silicate on known antifungal compounds and critical enzymes of the pathways elemental for systemic resistance inducers, so as to evaluate the potential for using them as alternatives to or in conjunction with, copper fungicides in the control of Anthracnose in avocado fruit. The study included storage temperature and time variations, to take account of the logistics in shipping avocado fruit to distant markets. Pre- and postharvest applications of phosphoric acid and potassium silicate were used, and after harvest, fruit were either ripened at room temperature (22oC) without storage or stored for 28 days at temperatures of 5.5oC or 2oC before analysis. Concentrations of phenolics, activity of the enzyme phenylalanine ammonia lyase (PAL) and a known antifungal diene were determined in the fruit exocarp. Pre-harvest treatments of phosphoric acid showed that the highest phenolic concentration was found in fruit harvested 14 days after application for fruit stored at room temperature. For fruit stored at 5.5°C it was seen that as fruit softened, phenolic concentrations increased compared with hard fruit immediately after storage, with the highest increase noted for fruit harvested 7 days after application. When comparing the three storage temperatures, phenolic concentrations were enhanced most when fruit was stored at 2°C. Postharvest treatments showed a significant increase in phenolic concentrations for potassium silicate treated fruit stored at room temperature and 2°C when determined immediately after storage. Fruit stored at 5.5°C showed an increase in phenolic concentrations as it became softer. When considering PAL enzyme activity, it was found that postharvest treatments of both potassium silicate and phosphoric acid influenced enzyme activity, with potassium silicate having greater effects. Similarly, an increase in PAL activity was noted in the pre-harvest phosphoric acid treatment harvested 14 days after application for fruit ripened immediately as well as fruit stored at 5.5°C. Fruit stored at 2°C showed the highest PAL activity for fruit harvested 7 days after application. No results were obtained in the analysis of antifungal compounds for both pre- and postharvest treatments. However, it is suggested that the antifungal diene could follow similar trends to those found for phenolics. It is concluded that applications of both phosphoric acid and potassium silicate do create changes in phenolic concentrations and the activity of the enzyme PAL which is involved in the synthesis of phenolic compounds known to possess antifungal properties. It is therefore possible that phosphoric acid and potassium silicate may be used as part of an integrated programme for Anthracnose control, and should be tested as potential alternatives for high volume copper-based fungicides.
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    Some aspects of cold storage of 'Fuerte' avocados (Persea americana Mill.) grown in the Natal midlands.
    (1995) Donkin, Derek John.; Wolstenholme, B. Nigel.; Cutting, Jonathan Garth Melville.
    The South African avocado industry is largely export orientated and export by sea to European markets necessitates cold storage for up to 4 weeks at temperatures around 5.5°C. Avocado fruit is subject to chilling injury which is manifested as mesocarp discolouration, and pitting and blackening of the rind. Of the South African cultivars exported, 'Fuerte' is the most susceptible to chilling injury, and accounts for > 50 % of avocado exports. A number of temperature regimes where temperature was reduced in a step-wise fashion from 8.5 or 7.5°C to 4.5 or 5.5°C during 3 to 5 weeks of storage were tested weekly throughout the 1993 and 1994 'Fuerte' harvesting seasons in the Natal Midlands (a cool mesic subtropical area), in attempt to find cold storage temperature regimes which would minimise chilling injury. No definite trends with regard to certain temperature regimes resulting in fruit with less chilling injury were evident. Overall, stepped down temperature regimes produced fruit of quality no better than storage for 5.5°C for 4 weeks. There was no significant difference in concentration of total phenolics in 'Fuerte' fruit mesocarp throughout the 1994 harvesting season (P < 0.05). Levels of ethylene evolution during 4 weeks of storage at 7.5 and 5.5°C ranged from 0 to 5 µl.kg(-l).h(-l), and peaked at 109 and 75 µl.kg(-l).h(-1) in fruit stored at 7.5 and 5.5°C respectively at room temperature on removal from cold storage. Rapid moisture removal from 'Fuerte' fruit after harvest and before cold storage by placing the fruit in glass jars to which a suction of -75 kPa was applied, resulted in increased susceptibility to external chilling injury, the severity of which was proportional to the amount of moisture removed from the fruit. Pre-storage heat treatments with a view to decreasing sensitivity of fruit to cold storage were carried out on 'Fuerte' fruit. Dry heat and warm water baths at temperatures of 36 to 40°C caused rind blackening of varying severity, depending on temperature and duration. Vapour heat treatments at temperatures of 36 to 48°C for 10 min to 48 h also caused rind blackening, with the exception of 10 min at 48°C and 1.5 and 3 h at 40°C which produced fruit of higher overall quality after 4 weeks of cold storage at 3.5°C than fruit not heat treated. These treatments however, could not be repeated in 1994 to confirm the results obtained as the harvesting season was over by the time the trial was completed.