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Doctoral Degrees (Genetics)

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    Identification of arthropods of forensic importance during cold and warm seasons in KwaZulu-Natal Province of South Africa.
    (2021) Tembe, Danisile.; Mukaratirwa, Samson.
    Forensic entomology is the study and use of insects and other arthropods in forensic investigations associated with death, abuse and neglect of both humans and animals. Although there has been an increased interest in forensic entomology and its application in predicting post-mortem interval (PMI) amongst other issues in many developed countries, the results cannot be extrapolated beyond the countries/regions of study since the arthropods species spectra may vary with region and geographical conditions. The present study aimed to determine the arthropod species of forensic importance found during different stages of decomposition of sheep (Ovis aries) and pig (Sus scrofa domesticus) carrion during the warm and cold season in KwaZulu-Natal province of South Africa. A scoping review was conducted to determine the state of knowledge of forensic entomology research and application in southern Africa. To determine the arthropod species associated with sheep and pig carcass during different stages of decomposition, two medium sized Large-White pigs and two medium sized Merino sheep were humanely killed and used for the cold and warm season. Adult arthropods found on and around the carcasses during different stages of decomposition were collected and identified using combined morphological identification keys and molecular technique based on the mitochondrial gene. The review showed that arthropod species that were found on a decomposing carcass could be useful in the estimation of PMI and provided clues in cases of criminal investigations. The review also confirmed the scarcity of forensic entomology research, and its application in southern Africa. Experimental results from this study showed that dipteran flies from the families Calliphoridae, Muscidae and Sarcophagidae were the first to colonize the sheep and pig carcasses during both warm and cold seasons. These include species of Chrysomya marginalis, Ch. putoria, Ch. albiceps, Ch. chloropyga, Lucilia cuprina, Musca domestica and Sarcophaga calcifera. On the sheep carcasses, Ch. marginalis, Ch. albiceps and M. domestica were the most dominant fly species, contributing 63.2 % of the collected flies in the warm season, and 68.9 % in the cold season. Colonization by coleopterans during the warm season started as early as the fresh stage with Dermestes maculatus, Thanatophilus micans and Onthophagus crassicollis. In the cold season these same beetle species were collected from the bloated stage of the sheep carcass. On the pig carcasses, Ch. marginalis (n = 111), Ch. albiceps (n = 99) and M. domestica (n = 131) were the most abundant species during the warm season. The same species were the most abundant species in the cold season (n = 55), (n = 34) and (n = 81) respectively, although in lower numbers than the warm season. Among the collected Coleoptera species, D. maculatus (n = 112) and N. rufipes (n=62) were the most abundant species found on the carcass during the warm season and the same species were the most abundant species in the cold season (n = 66) and (n = 48) respectively. In the warm season Dermestes maculatus was recorded on the pig carcass during the fresh stage and persisted on the carcass until the last of decomposition. However, in the cold season Dermestes maculatus was first recorded on the carcass during the active stage of decomposition. Molecular analyses confirmed the identification of twelve (12) arthropod taxa collected from both sheep and pig carcasses during the cold season. Results showed that 11/12 arthropod species were common in both sheep and pig carcasses, with exception to Onthophagus sp. and Atherigona soccata species which were unique to sheep and pig carcasses respectively. However, during the warm season, the sheep carcass attracted more (n=13) taxa as compared to the pig carcass. The variation in the arthropod was due to the presence of Onthophagus sp. which was also unique to the sheep carcass during this season. Furthermore, there was an addition of a beetle species Hycleus lunatus, which was collected from both sheep and pig carcasses but unique to the warm season. This study generated important information on the endemic arthropod species that are of forensic importance KwaZulu-Natal province. The arrival time and association of arthropods species with different stages of decomposition during the warm and cold season highlighted their value in estimating the PMI in forensic investigations in the locality of KwaZulu-Natal province. The studied arthropods can potentially be useful in the estimation of PMI and other cases of criminal investigations. The seasonal variations in abundance of both Diptera and Coleoptera in the two seasons seemed to indicate influence of seasons which subsequently influenced temperature. It is recommended that similar studies be conducted at other geographical locations of South Africa with a different ecological system to build a database of dipteran and coleopteran species of forensic importance which are endemic in these areas.
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    Quantifying redox signals: a tool to investigate adaptive responses to oxidative stress.
    (2024) Lind, Diane Justine.; Pillay, Che Sobashkar.
    In the context of redox biology's oxidative eustress/distress model, the contrasting roles of ROS, such as hydrogen peroxide, under normoxic and toxic oxidative stress conditions has been more clearly elucidated. However, a fundamental question in the field has been understanding how dynamic redox signalling processes contribute to balancing the cellular response to oxidative eustress/distress.We employed a quantitative approach, evaluating redox signals based on area under the curve (AUC), signal amplitude, time, and duration. These parameters allowed us to investigate how dynamic profiles changed in response to various oxidants across three distinct experimental contexts. First, quantifying the dynamic response of the Tpx1/Pap1 redox-regulated pathway in Schizosaccharoymyces pombe revealed a graded signal and transcriptional response to input peroxide concentrations. Secondly, we applied this method to establish the upper and lower limits of detection for the redox probes HyPer7 and roGFP-TSA2/Tpx1 in Saccharomyces cerevisiae and S. pombe, providing a set of criteria for improved selection and comparison of probes. Thirdly, we quantitatively evaluated the dynamic response of the mammalian NRF2/Keap1 pathway following pre-exposure to DEM and tBHQ. Our findings revealed that, unlike the Tpx1/Pap1 pathway, this pathway showed that the timing of oxidant exposure influenced the NRF2 response. These metrics have therefore provided useful insights to several different pathways in both experimental and analytical contexts. Importantly, these measures allow for further exploratory questions to be asked and provide a fundamental framework for assessing dynamic responses of redoxregulated pathways associated with cell physiology and disease. Iqoqa. Esimweni semodeli i-redox biology's oxidative eustress/distress, izimo ezinokuphikisana ze-ROS, njengehydrogen peroxide, ngaphansi kwezimo zenormoxic kanye netoxic oxidative stress zicaciswe bha. Kepha-ke umbuzo omkhulu esifundweni owokuqonda ukuthi ngabe izinhlobo ezikhombayo zedynamic redox zibamba liphi iqhaza ekubhalansiseni ukuphendula kweselula ku-oxidative eustress/distress. Sasebenzisa indlela yekhwalithethivu, ukuhlola izimpawu zeredox sisusela endaweni engaphansi kwejika (AUC), ukukhombisa, ubukhulu, isikhathi kanye nobude besikhathi. La mapharamitha asivumela ukuba sihlole ukuthi ngabe amaphrofayli edynamic ashintsha kanjani kuma-oxidants ahlukene ezindaweni ezintathu ezahlukene lapho kuhlolwa khona. Okokuqala ukubala iresponse yedynamic ye-Tpx1/Pap1 redox-regulated pathway ku-Schizosaccharoymyces pombe yaveza izinkomba ezihlukanisiwe kanye nokuphendula okutranscriptional kokufakiwe kwe-peroxide concentrations. Okwesibili sasebenzisa le ndlela ukuthola imikhawulo ephezulu nephansi yokuhlola iredox probes HyPer7 kanye ne-roGFP-TSA2/Tpx1 no-Saccharomyces cerevisiae ne-S. pombe, ukuhlinzeka ngesethi yendlela yokukhetha okuthuthukile kanye nokuqhathaniswa kwamaprobes. Okwesithathu sahlola ngokwekhwantithethivu ukuphendula kwe-dynamic ye-mammalian NRF2/Keap1 pathway kulandela ukuvuleleka ngaphambilini ku-DEM kanye ne-tBHQ. Imiphumela yethu yaveza ukuthi kungafani nomgudu i-Tpx1/Pap1, lo mgudu wakhombisa ukuthi isikhathi sokuvuleleka kwe-oxidant sinomthelela ekuphenduleni kwe-NRF2. La mamethrikhsi ahlinzeke ngolwazi lwemigudu eyehlukene ezimweni zokuhlola kanye nokuhlaziya. Okusemqoka, lezi zindlela zivumela eminye imibuzo yokuhlwaya ukuba ibuzwe bese ihlinzeka uhlaka olusemqoka lokuhlola ukuphendula kwedynamic yemigudu ye-redox-regulated eyamaniswa namaseli okusebenza komzimba kanye nezifo.