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Doctoral Degrees (Biological Sciences)

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Browsing Doctoral Degrees (Biological Sciences) by Author "Beckett, Pichard Peter."

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    Cathodic water invigoration of deteriorated orthodox seeds – implications on subsequent plant growth.
    (2020) Fatokun, Kayode.; Beckett, Pichard Peter.; Varghese, Boby.; Pammenter, Norman William.
    Orthodox seeds deteriorate even when kept under the best of conditions leading to loss of germination, poor seedling growth. In this study, a novel approach via the use of cathodic water, an electrolysed form of calcium magnesium solution, was used to invigorate fresh and controlled deteriorated seeds of wild (Bolusanthus speciosus (Bolus) Harms, Combretum erythrophyllum (Burch.) Sond., Erythrina caffra Thumb.) and agricultural (Pisum sativum L., Cucurbita pepo L.) species. Other treatment solutions investigated alongside cathodic water were un-electrolysed calcium-magnesium solution and deionized water. Fresh seeds of the test species were controlled deteriorated to 50% germination (P50) at 40°C and 100% relative humidity. Thereafter, the seeds were invigorated with the treatment solutions. Fresh and un-primed controlled deteriorated seeds served as the control. In addition, to study the mechanism of invigoration, the effects of priming on the membrane stability index (MSI), amylase activities, lipid peroxidation products malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) were investigated. The effects of priming on the reactive oxygen species (ROS) scavenging enzymes superoxide dismutase, and catalase and DNA (concentration and purity). All priming treatments improved germination, emergence, growth parameters and subsequent seedling photosynthesis relative to the unprimed seeds. In general, cathodic water was most effective at invigorating seeds in the all test species. Also, controlled deteriorated seeds benefitted more than the fresh seeds treatments. Analyses of the lipid peroxidation products and antioxidant enzyme activities in invigorated seeds provided support for the hypothesis that the effectiveness of cathodic water in the invigoration of debilitated orthodox seeds derive from its ability to act as a potent antioxidant. This study, which is a novel approach at bringing the concept of electrochemistry into germplasm conservation via the use of cathodic water, has also confirmed the efficacy of cathodic water in invigorating debilitated seeds. This is especially critical for seeds containing traits that may be under the threat of being lost due to various reasons. The current study reinforces the strong potential of cathodic water in the recovery of aged germplasm and improved yield of orthodox seeded species.