Browsing Masters Degrees (Virology) by Title
Now showing items 8-27 of 33
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The development, optimisation and comparison of various virological assays and their uses in antiviral assessment of compounds wih potential anti-HIV activity.
(2009)The development and optimization of anti-viral screening methods are essential to develop newer more effective, treatments against HIV. The XTT method is a widely described method for antiviral screening. Both continuous ... -
The differential influence of HIV-1 subtype C,nucleoside analog resistance mutations: K65R, A62V, S68N and Y115F susceptibility to tenofovir.
(2019)The use of Tenofovir Disoproxil Fumerate (TDF) for the treatment of HIV-1 infection has been recommended for the first-line as well as a second-line antiretroviral regimen in South Africa, due to its high antiretroviral ... -
The epidemiology of dual HIV infection in the KwaZulu-Natal Anti-Retroviral Roll-out Programme.
(2007)KwaZulu-Natal has the highest prevalence of HIV in South Africa. The prevalence of dual infection in a normal-risk population in this region is unknown. Dual HIV infection has important implications for diagnosis, treatment ... -
Functional characterization of human immunodeficiency virus type 1 (HIV-1) subtype C transmitted/founder (T/F) viruses long terminal repeat (LTR) variants and association with disease outcome.
(2020)Background: The persistence of latent viral reservoirs is a major roadblock to human immunodeficiency virus type 1 (HIV-1) cure development. Latent reservoirs harbour transcriptionally silent yet replication competent ... -
Genetic and functional diversity of central nervous system (CNS) derived Human Immunodeficiency Virus type 1 (HIV-1) tat from Tuberculous Meningitis (TBM) patients.
(2018)INTRODUCTION Human immunodeficiency virus type 1 (HIV-1) transactivator of transcription (tat) is a regulatory gene that encodes the transactivator of transcription Tat protein. The Tat effectively increases the activity ... -
Genetic characterization of viral blips in patients following suppressive HIV ART.
(2021)Antiretroviral therapy (ART) has resulted in the decline of HIV-related mortality worldwide. On initiating treatment, most patients can suppress plasma viral RNA to undetectable levels (<20 copies/mL). Patients on ART ... -
Identifying novel transcriptional regulatory elements of HLA-A alleles through the evaluation of the 5’ un-translated region sequences.
(2020)Sub-Saharan Africa holds approximately half the population living with human immunodeficiency virus (HIV) in the world (~19.6 million), of which around 7.2 million cases are found in South Africa. Although antiretroviral ... -
Identifying the cellular HIV-1 reservoir in lymph nodes of antiretroviral therapy suppressed individuals.
(2019)HIV-1 infection is suppressed but not cured in the face of antiretroviral therapy (ART). Pinpointing the cellular HIV-1 reservoir, which allows HIV-1 to persist, is key to the eradication of the virus. Lymph nodes are known ... -
Impact of p2/NC cleavage site polymorphisms on HIV-1 subtype C viral fitness.
(2012)Subtype C accounts for the majority of HIV infections and in South Africa, is the dominant subtype. The Gag cleavage sites of subtype C viruses show a high degree of natural variation compared to subtype B and group M ... -
Implementation of an efficient sample pooling strategy for high throughput diagnostic testing of Severe Acute Respiratory Syndrome Corona Virus-2.
(2022)The rapid identification and isolation of infected individuals remains a key strategy for controlling the spread of SARS-CoV-2. Frequent testing of populations to detect infection early in asymptomatic or presymptomatic ... -
In vitro testing of the predicted viral fitness landscape for the HIV-1 Nef protein.
(2015)Abstract available in PDF file. -
The influence of HIV-1 genomic target region selection and sequence length on the accuracy of inferred phylogenies and clustering outcomes.
(2017)To improve the methodology of HIV-1 cluster analysis, we addressed how analysis of HIV-1 clustering is associated with parameters that can affect the outcome of viral clustering. The extent of HIV clustering, tree ... -
Innovative and affordable HIV-1 drug resistance testing for resource limited settings.
(2022)Background: HIV drug resistance (HIVDR) remains a major threat to achieving sustainable viral suppression on antiretroviral treatment (ART). Most countries including those in resource limited settings (RLS) have adopted ... -
Investigating the impact of HIV infection on ILC3s in human lymph nodes.
(2022)People living with HIV (PLWH) develop extensive fibrosis and collagen deposition throughout their lymphoid tissues not reversed by antiretroviral therapy (ART). Innate lymphoid cells (ILCs) play essential roles in tissue ... -
An investigation into the serological and molecular diagnosis of Jaagsiekte Sheep Retrovirus (JSRV)
(2005)The Jaagsiekte Sheep Retrovirus (JSRV), an exogenous type B/D-retrovirus with about 10-15 endogenous counterparts in all normal sheep genomes, causes Jaagsiekte (JS) or ovine pulmonary adenocarcinoma (OPA), a contagious ... -
An investigation of virologic failure and the spectrum of drug resistance mutations in a paediatric ART programme in rural KZN, SA.
(2014)Background Better understanding of drug resistance patterns in HIV-infected children on antiretroviral therapy (ART) is required to inform public health policies in high prevalence settings. The aim of this study was to ... -
Measuring HLA-B allele expression across differential cell types.
(2018)Background: The human leukocyte antigen (HLA) region has shown to have the strongest disease associations and recent studies have shown that expression levels of these HLA molecules play a major role in the clinical course ... -
Minority HIV-1 drug resistance mutations in patients failing highly active antiretroviral therapy (HAART).
(2014)Abstract not available. -
Molecular diagnosis and typing of HTLV-I in KwaZulu-Natal.
(1998)Two areas of the HTLV-I genome were targeted for an in-house molecular diagnostic test, namely the pol and env regions. The pol primers proved the most sensitive (100%)and specific (100%). Amplification using the env primer ...