Now showing items 1-10 of 10
The development of assays for atractyloside and its localisation in rat tissue.
An extract of the tuber of Callilepis laureola is regarded as the source of a powerful therapeutic agent, known as Impila. Its use is associated with fatal hepatic and renal necrosis, the renal toxin being atractyloside ...
A study of proteinases of invasive cells using cryoultramicrotomy and immunogold labelling.
This study forms part of an investigation into the possible relevance of the lysosomal proteinases, cathepsins B, H, Land D, in cancer cell invasion. In this study, the main technique adopted was the Tokuyasu "cryo" ...
A study of the proteinase, cathepsin L, in the context of tumour invasion.
The proteinase, cathepsin L, has been strongly implicated in the processes of tumour invasion and metastasis. A new purification method, three-phase partitioning, characterised in terms of the parameters which affected its ...
Making sense of mixtures : chromatographic separations of plant, insect and microbial biomolecules.
No abstract available.
Trypanopain : a possible target for anti-trypanosomal agents?
The protozoan parasite Trypanosoma brucei brucei causes nagana in cattle and is a widely used model for human sleeping sickness. The major lysosomal cysteine proteinases (trypanopains) of African trypanosomes may contribute ...
A serine oligopeptidase from African Trypanosomes.
Protozoan parasites of the genus Trypanosoma are responsible for chronic and widespread disease in livestock and humans in Africa. This study describes the purification and characterisation of a serine oligopeptidase ...
Studies on a multicatalytic, protease complex from Trypanosoma brucei brucei.
Subcellular fractionation (together with immunocytochemical localisation studies) showed that the parasite Trypanosoma brucei brucei possesses a multicatalytic protease complex (MCPTb). This complex is predominantly ...
Studies on acid phosphatases of Trypanosoma congolense.
Bloodstream forms of African trypanosomes, which endocytose macromolecules exclusively through their flagellar pockets, contain an acid phosphatase (AcP) activity in this organelle. In the present thesis, AcP activity ...
Cloning of the promoter regions of Trypanosoma brucei and Trypanosoma congolense cysteine protease genes.
Trypanosoma brucei and T. congolense are protozoan parasites that infect humans, domestic livestock and wildlife in Africa. These parasites undergo complex morphological and biochemical changes, during the various stages ...
Redox properties of cathepsin B in relation to its activity in vivo.
The main site for protein degradation along the endosomal pathway is believed to be the late endosome. Lysosomes are thought to be storage organelles that, when necessary, inject proteases into the late endosome. It was ...