Studies on Lecanicillium Muscarium as a Mycoparasite of the soybean rust fungus, Phakopsora Pachyrhizi.
Havugimana, Jean Pierre.
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Soybean rust (SBR) is the most damaging foliar disease in many soybean growing areas of the World. Fungicide sprays seems to be the only method available for SBR control. An alternative to fungicides is needed, considering that some Phakopsora pachyrhizi strains have shown resistance to triazole fungicides. The main objective of this chapter was to isolate and identify a Lecanicillium spp. that could be used for SBR control. An isolate of Lecanicillium spp strain Nesta-08 was isolated from the Assagay Coffee Farm, Cato Ridge, KwaZulu-Natal, South Africa, where it was observed parasitizing Hemileia vastatrix, the causal agent of the coffee rust. The fungus was isolated on Sabouraud dextrose yeast extract agar (SDYA) and the pure culture obtained was subcultured onto potato dextrose agar (PDA). Under light microscopy, conidia were found to be ovoid to ellipsoidal. Under scanning electronic microscopy, the size of conidia ranged between 1.9-3.9μm. Based on nBLAST using ITS and ITS4 sequences, the isolate Nesta-08 presented 99.8% similarity to strain CBS 318.70B of L. muscarium. Based on the DN3 gene, isolate Nesta-08 shows a common ancestor with L. muscarium at a low bootstrap value (58-59). Based on DN1, isolate Nesta-08 shows a common ancestor with L. muscarium and is supported with a high bootstrap value of 94. Based on morphological and molecular studies the isolate was identified as Lecanicillium muscarium. It was deposited into the National Collection of Fungi with an accession number PPRI 13715.