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dc.contributor.advisorLin, Johnson.
dc.contributor.advisorMehlo, Luke.
dc.creatorMbambo, Zodwa.
dc.date.accessioned2014-09-22T06:49:18Z
dc.date.available2014-09-22T06:49:18Z
dc.date.created2013
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10413/11247
dc.descriptionPh.D. University of KwaZulu-Natal, Durban 2013.en
dc.description.abstractClimate change, shrinking arable land, burgeoning population and malnutrition have made all aspects of crop improvement a critical issue. Of these, nutritional quality of crops is perhaps one of the most important aspects. Most cereals consumed in marginal agro-ecological zones of Africa, for example sorghum and maize are impoverished nutritionally. Given therefore the sole reliance on and the levels of consumption per day of such staples (up to 450 g/day), it is clear that most people cannot obtain the recommended daily allowance (RDA) for many nutrients including fibre, edible oil, protein, vitamins and mineral elements. In this thesis, the development of a sorghum mutant population using gamma irradiation and the subsequent employment of various analytical techniques to unravel multiple mutant traits with a significant positive impact on nutritional enhancement in sorghum is described. Protein analysis revealed a mutant designated SY accumulating (at the time) the highest ever reported amount of free lysine (21.6 g/100g) and other essential amino acids and that these changes were associated with induced protein polymorphisms. Adaptation of proton induced x-ray emission (PIXE) for the spatial profiling of the distribution of 9 elements in sorghum seed tissue allowed for the discovery of mutants with variations in the concentrations and distribution of these elements. The observed changes included enhanced or diminished accumulation of elements in preferential accumulation tissues and entire changes in cellular localisation. The locations within a cell and the quantities of an element are often critical determinants of bioavailability. The accumulation of multiple mutations affecting multiple nutritional traits in individual mutant sorghum clearly indicates the versatility of gamma irradiation induced mutations in addressing multiple nutritional challenges of sorghum. This desirable phenomenon was further demonstrated by electron microscopic analysis of starch granules and protein bodies across the mutants. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed changes in size, shape, ultra-structure and packed cell volumes of seed protein- and starch bodies. Induced mutation had a major effect on the protein body structure which in turn resulted in changes to protein digestibility. High digestibility mutants had a unique dense protein matrix with dark inclusions. However, improved protein quality traits were also associated with floury endosperm texture. Since endosperm texture is an important grain quality attribute and plays a major agronomic role, it is important to ensure that future work focuses on improving grain hardness. The mutants obtained in this study are therefore a valuable germplasm source for sorghum breeding and present real opportunities for addressing nutritional challenges of sorghum.en
dc.language.isoen_ZAen
dc.subjectSorghum--Genetics.en
dc.subjectSorghum--Breeding--Africa.en
dc.subjectSorghum--Mutation breeding--Africa.en
dc.subjectSorghum.en
dc.subjectMutagenesis.en
dc.subjectTheses--Microbiology.en
dc.titleThe impact of induced mutations on key nutritional and agronomic traits of sorghum.en
dc.typeThesisen


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