Georgiev, Ivelin S.Doria-Rose, Nicole A.Zhou, Tongqing.Do Kwon, Young.Staupe, Ryan P.Moquin, Stephanie.Chuang, Gwo-Yu.Louder, Mark K.Schmidt, Stefan.Altae-Tran, Han R.Bailer, Robert T.McKee, Krisha.Nason, Martha.O'Dell, Sijy.Ofek, Gilad.Pancera, Marie.Srivatsan, Sanjay.Shapiro, Lawrence.Connors, Mark.Migueles, Stephen A.Morris, Lynn.Nishimura, Yoshiaki.Martin, Malcolm A.Mascola, John R.Kwong, Peter D.2013-12-122013-12-1220122012Georgiev, I.S., et al. 2013. Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization. Science. 340(6133) pp. 751-756.0036-8075http://dx.doi.org/10.1126/science.1233989http://hdl.handle.net/10413/10217Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1–neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined “neutralization fingerprints” for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1–infected donors and a chimeric siman-human immunodeficiency virus–infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.enHIV infections--Immunology.HIV antibodies.HIV (Viruses)--Analysis.Neutralizing antibodies.Peer reviewed journal article