Browsing Masters Degrees (Biochemistry) by Issue Date
Now showing items 1-20 of 127
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Synthesis of DNA - protein conjugates and a preliminary study of their interaction with eukaryotic cell receptors.
(1986)Thymidine oligomers were chemically synthesised and linked to available amino functions of transferrin in alternative orientations: (a) A CMP residue attached to the 3' end of (pT)₁₀ with terminal deoxynucleotidyl transferase ... -
Studies on the preparation and interaction of modified transferrin-DNA complexes with HeLa cells.
(1986)The correction of human genetic disorders by transfer of genetic material to cells is under intensive investigation in a number of 1aboratories. One possible way of trying to achieve the transfer of nucleic acid is ... -
Preparation of chemically modified transferrin proteins and an investigation of their reactions with DNA and other nucleic acids.
(1986)The molecular biology of human genetic disorders is under intensive investigation at present. In those cases where the disorder is clearly defined in terms of altered gene structure, possibilities may exist for the correction ... -
Canine anti-endotoxin immunotherapy in cranial mesenteric arterial occlusion shock and canine parvovirus disease endotoxaemia.
(1986)Endotoxin (LPS, lipopolysaccharide) forms an integral part of the outer cellular membrane of gram negative bacteria (GNB). The canines' intestine always contains large amounts of GNB, and hence LPS. If these GNB with ... -
Cloning of the promoter regions of Trypanosoma brucei and Trypanosoma congolense cysteine protease genes.
(1997)Trypanosoma brucei and T. congolense are protozoan parasites that infect humans, domestic livestock and wildlife in Africa. These parasites undergo complex morphological and biochemical changes, during the various stages ... -
Redox properties of cathepsin B in relation to its activity in vivo.
(1999)The main site for protein degradation along the endosomal pathway is believed to be the late endosome. Lysosomes are thought to be storage organelles that, when necessary, inject proteases into the late endosome. It was ... -
Role of neutrophil matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteases-1 (TIMP-1) in the killing of microorganisms.
(2003)Microorganisms may evade killing by neutrophils (PMNs) by altering signal transduction and hence phagosome maturation. Secreted, active matrix metalloproteinases (MMPs) appear to be required for PMN killing of pseudomonas ... -
Elemental composition in monocytes in response to anti-malarial drugs and hemozoin.
(2003)Every year there are approximately 300 million new cases of malaria with 2 million deaths. The majority of deaths occur in African children between the ages of 1 and 4 years and are caused by the parasite Plasmodium ... -
Cloning and recombinant expression of a 822 bp region of a Pf403 Plasmodium falciparum gene.
(2003)Malaria is a devastating parasitic disease in humans caused by species in the genus Plasmodium. With over 100 million cases and at least 1.5 million fatalities each year, the disease accounts for 4-5% of all fatalities ... -
Coupling dyes to chicken IgY antibodies for the development of immunodiagnostic tests.
(2003)The aim of this study was to develop a highly simplified, sensitive and specific malarial diagnostic test at the lowest possible cost. Initial work and optimisation of procedures was achieved with chicken antibodies by ... -
Investigation of chlorophyll and stomatal chloroplast content in diploid and tetraploid black wattle (Acacia mearnsii de Wild).
(2004)Black wattle (Acacia mearnsii) is one of South Africa's leading commercial exotic species comprising nearly seven percent of South African forestry plantations. The planting of black wattle has become increasingly popular, ... -
Assessment of genetic diversity and DNA fingerprinting of the Cape parrot (Poicephalus robustus) using randomly amplified polymorphic DNA (RAPD)
(2004)The Cape parrot (Poicephalus robustus) is South Africa's only endemic parrot. It has become increasingly rare in recent years, with fewer than 500 birds left in the wild, and is now regarded as endangered. Possible factors ... -
Dye-protein interactions : protein staining and dye-IgY, dye-dextran-IgY complexes for antigen detection.
(2004)In order to develop a cheaper alternative to the conventional enzyme-linked immunosorbent assay system, application of dye molecules as labels in immunoassay was investigated in this study. This chromogenic dye-antibody ... -
The influence of ionic strength on the kinetics of selected enzymes.
(2005)pH studies are used to gain insight into chemical mechanisms of enzyme catalysed reactions. However, perhaps the most important practical point that is often overlooked in pH studies is control of the ionic strength of ... -
Induction of autoantibodies to cathepsin L as a step towards an anti-cancer vaccine.
(2005)Cancer is a disease that is caused by mutations in somatic cells. Metastasis is the major cause of death from cancer and often complicates treatment. Malignant tumours secrete degradative enzymes such as cathepsin L which ... -
Protease distribution in J774 macrophages
(2007)Cathepsin, matrix metalloproteinase (MMP) enzyme and tissue inhibitor of MMP (TIMP) distribution in J774 mouse macrophages has not been comprehensively studied. The distribution and vesicle regulation, trafficking and ... -
Development of a taxonomy for visual literacy in the molecular life sciences.
(2007)The use of external representations (ERs) such as diagrams and animations in science education, particularly in the Molecular Life Sciences (MLS), has rapidly increased over the past decades. Research shows that ERs have ... -
Characterization of the immunity factor in producer self protection against Leucocin A.
(2008)Lactic acid bacteria produce pediocin-like bacteriocins designated as Class Ha. These antimicrobial peptides are antagonistic against Listeria monocytogenes and other closely related Gram-positive bacteria Self-protection ... -
Evaluation of congopain and Oligopeptidase B as anti-disease vaccines for African Trypanosomiasis.
(2008)The protozoan parasite Trypanosoma congolense is one of the aetiological agents of African animal trypanosomiasis that is transmitted by the tsetse fly. The parasite causes nagana in animals and affects livestock throughout ... -
Endoplasmic reticulum associated degradation (ERAD) overflow pathways.
(2008)Accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes numerous human pathologies. Biochemical evidence suggests that soluble misfolded proteins are retrotranslocated out of the ER, via the endoplasmic ...