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Masters Degrees (Biological Sciences)

Permanent URI for this collectionhttps://hdl.handle.net/10413/12380

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    Morphological and molecular characterization of Fasciola hepatica and Fasciola gigantica phenotypes from Mpumalanga and KwaZulu-Natal provinces of South Africa.
    (2020) Haridwal, Sayurika.; Mukaratirwa, Samson.
    Fascioliasis is a food- and waterborne disease. It is one of the most common helminthic infections in domesticated ruminants. The disease is caused by liver flukes, Fasciola hepatica and Fasciola gigantica. Increased attention has been geared toward studying these flukes due to their ever-expanding geographical distribution, enormous economic impact, increased human infections, increased resistance to treatment and the existence of hybrid forms. Both these species are co-endemic in the Mpumalanga and KwaZulu-Natal provinces of South Africa and even though, hybrids have been reported in other areas where both species exist it has not been attempted in South Africa. Therefore, this study was conducted to determine the existence of Fasciola hybrids in South Africa using morphological and molecular characterization. A total of 71 flukes were collected from naturally infected cattle slaughtered at abattoirs located in Enhlazeni and Nelspruit in Mpumalanga province and Pietermaritzburg in KwaZulu-Natal province of South Africa as well as control samples from Zimbabwe (Bulawayo abattoir) where only F. gigantica exist. The samples were categorized morphologically as either F.hepatica, F.gigantica, or Fasciola sp. The morphometrics (body length, body width, and length/width) were analyzed through a PCA and produced three distinct groups. A one-way ANOVA indicated that the length and length/width could be used to differentiate the species (P < 0.05) and the width was not useful in differentiating the species (P > 0.05). Molecular analysis based on ITS-1/5.8S/ITS2 marker showed that specimens morphologically identified as Fasciola sp were F.gigantica, with one sample morphologically identified as F.gigantica was molecularly identified as Fasciola sp. Similar results were observed with the CO1 marker, however, one sample came up as unknown, this sample however, formed a well-supported sister clade to F. gigantica. . It was also observed that aspermatic specimens are not only limited to hybrids, as some individuals that were molecularly identified as F. hepatica lacked sperm in their seminal vesicles. This study confirms species identification of F. hepatica and F.gigantica cannot be solely based on morphological characters where both these species are co-endemic. This was also the first study to report the existence of hybrid Fasciola spp. in South Africa.
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    Morphology, phytochemistry, and medicinal properties of South African Mangifera indica L. leaves for summer and winter seasons.
    (2021) Maharaj, Arvish.; Naidoo, Yougasphree.; Dewir, Yaser Hassan.
    Herbal preparations of plants continue to present mankind with novel remedies as many of these plants contain important secondary metabolites. Plant species of the family Anacardiaceae are rich in bioactive phytochemicals. Mangifera indica (Anacardiaceae) is an introduced and naturalised species to South Africa. Herbal use of this plant has not been fully documented; however, it is used in traditional medicine. This study aimed at characterizing the morphology, phytochemistry, and biological activity of Mangifera indica leaves harvested in winter and summer. The foliar biology of the plant was conducted by various microscopy techniques such as stereo- and Scanning electron microscopy. The length and diameter of the different trichome types were measured using ImageJ. The non-glandular trichome lengths range between 70 - 200 μm. The peltate gland trichomes consist of 2 rows of 8 oblong cells each with a size ranging from 32- 48 μm. Morphological observations using stereo- and SEM revealed the presence of non-glandular trichomes with cuticular warts and glandular peltate trichomes on the leaves of Mangifera indica. Transmission electron micrographs showed the presence of numerous mitochondria, starch grains, plastoglobuli, and plastids. The results for summer and winter leaves resembled somewhat similar-to-identical morphological characteristics on all fronts. For the phytochemical and biological assays, this study aimed to investigate some of the phytochemical and biological properties using different solvents (hexane, chloroform, and methanol) for extraction of the leaves of Mangifera indica for the summer and winter seasons. Preliminary phytochemical screening for the hexane, chloroform and methanolic extracts was done using a reflux extraction apparatus to uncover the presence of different metabolites and the anti-oxidant screening was done by the radical scavenging activity, which was established using the 2,2-diphenyl-1-picrylhydrazyl assay. Potent radical scavenging activity was exhibited for both summer and winter seasons with hexane and methanolic extracts for summer (IC50 of 19.53 μg/mL and 12.71 μg/mL respectively) and winter (22.32 μg/mL and 14.35 μg/mL respectively) in comparison to the control ascorbic acid which produced an IC50 of 3.20 μg/mL. The summer extracts had better radical scavenging IC50 capacity than winter extracts. The antibacterial activity of the methanolic leaf extracts for summer and winter of Mangifera indica were evaluated against the bacterial species: Gram-negative Escherichia coli (ATCC 25922) and Gram-positive: Staphylococcus aureus (ATCC ATCC 43300). For S. aureus (ATTC 43300), the summer crude extract displayed lower antibacterial activity than the control streptomycin, the summer extracts had a zone of inhibition of 14.17 mm while streptomycin had a 16.67 mm zone of inhibition. winter extracts had a zone of inhibition of 12 mm while streptomycin had a 13.67 mm zone of inhibition. For E. coli (ATCC 25922), the summer crude extract displayed higher antibacterial activity than the control gentamycin; the summer extract had a zone of inhibition of 18.05 mm while gentamycin had a 17.5 mm zone of inhibition. The winter extracts had a zone of inhibition of 8.5 mm. while gentamycin had a 14.5 mm zone of inhibition. Between seasons, summer had better antibacterial activity compared to winter for both Gram-positive and Gram-negative bacteria. Phytochemical screening showed the presence of phenols, flavonoids, tannins, and terpenoids, alkaloids, phytosterols, saponins, steroids, and carbohydrates. Potent radical scavenging activity was exhibited for the hexane and methanolic extracts for summer and winter, indicating that Mangifera indica is a potential source of medicinally important compounds. Antibacterial screening showed positive results with antibacterial properties for both summer and winter samples revealing its valuable biological activities. Summer overall performed better than the winter season. Future studies on this plant species are recommended to advance the use of indigenous herbal medicine or produce novel drug leads. To our knowledge, this study represents the first recent investigation in South Africa describing key foliar micromorphological features, phytochemicals, and biological activities of Mangifera indica L.