Masters Degrees (Soil Science)

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Environmental factors influencing the distribution hookworm infection in KwaZulu-Natal, South Africa [sic].
(1998) Mabaso, Musawenkosi.; Appleton, Christopher Charles.; Hughes, Jeffrey Colin.
The aim of this study was to investigate the occurrence of the soil transmitted parasitic nematode Necator americanus ("Old World" hookworm) in soils of different texture in KwaZulu-Natal. The key questions being asked were: (i) Is hookworm infection in KwaZulu-Natal confined to the coastal plain? (ii) Is there any association between hookworm prevalence and the different soil types in the province? (iii) Since several examples exist in the province of soil types on which hookworm is transmitted on the coastal plain, occurring inland, what is the status of infection in communities situated in these areas? (iv) What properties of soil are important in the transmission ecology of hookworm larvae? All available hookworm prevalence data of KwaZulu-Natal were mapped on Land Type maps of the province (Land Type Survey Staff, 1986). Several additional surveys were carried out to supplement this database. Faecal egg counts were obtained by the Formal-Ether Concentration Method and positive infections were confirmed as N. americanus by larval morphology after coproculture using the Harada-Mori Technique. Univariate analysis was carried out for significant associations between hookworm prevalence, altitude, climatic variables (rainfall and temperature) and soil type. The results showed that areas ≤ 150m above sea level (i.e. the coastal plain) support high prevalences (x ‾ = 45 %), and are characterised by low-clay textured soils, warm temperatures and relatively high rainfall. Areas > 150 m (i.e. inland) have low hookworm prevalences (x ‾ = 6 %), and are characterised by high-clay textured soils, cool temperatures and moderate rainfall. Hookworm prevalence also decreased southwards as climatic conditions (rainfall and temperature) become unfavourable, and the coastal plain also narrows in this direction. Multivariate analysis was done to determine which environmental factors combine best to provide favourable conditions for hookworm transmission. From the variables used, prevalence of infection was most significantly correlated with the mean daily minimum temperature for January followed by the mean number of rainy days for January. This points to the importance of summer conditions in the transmission of hookworm infection in KwaZulu-Natal. Moderate hookworm prevalences (x ‾ = 17.3 %) were found in the inland sandy areas, dropping to low prevalences (x ‾ = 5.3 %) in the surrounding non-sandy areas. The intensity-related data could not be significantly correlated with the environmental variables used in this study. The Spearman Correlation Coefficient was used to test for relationships between hookworm prevalence and soil variables. In the results, only the fine and medium sand fractions showed positive correlations with hookworm prevalence. Clay showed a significant negative correlation with hookworm prevalence. No significant correlations were found between soil pH or its organic matter content and hookworm prevalence. Age and sex related infection data could not be drawn into the analysis due to the small sample size of study localities.
An evaluation of the use of organic amendments to ameliorate aluminium toxicity and phosphorus deficiency in an acid soil.
(2000) Mokolobate, Motlogeloa Salathiel.; Haynes, Richard John.
The effects of the additions of some commonly-available organic residues to an acid, P-deficient soil (typical of those used by small-scale farmers in KwaZulu-Natal) on soil pH, exchangeable and soil solution AI, P availability and maize yield response was investigated in a number of laboratory and glasshouse experiments. The organic amendments used were ground-up grassveld residues, household compost, filter cake (a waste product from a sugar mill) and layer poultry manure. The soil used was a Hutton form (Farmingham series) (Rhodic Ferrasol, FAO). In an initial laboratory study, addition of all of the organic residues, at rates equivalent to 10 and 20 Mg ha¯¹, raised soil pH significantly and as a result there was a marked reduction in exchangeable AI concentrations. The increase in pH and decrease in exchangeable AI was more pronounced at the higher rate of addition and followed the order: poultry manure> filter cake> household compost> grass residues. The major mechanism responsible for the increase in pH was thought to differ depending upon the type of organic residue being considered. Whilst the relatively high content ofCaC0₃ was probably the main mechanism in the case of poultry manure and filter cake, the proton consuming ability of humic material probably predominated for household compost and decarboxylation of organic acids during decomposition was probably the main mechanism in the case of grass residues. Additions of organic amendments also decreased concentrations of total AI (AIT) in soil solution but the concentration of monomeric AI (AIMono) as estimated by pyrocatechol violet 60 sec. method, was unchanged or even increased. This latter effect was attributed to the high cation content of residues (particularly that of poultry manure) which increased soil salinity and exchangeable AI³⁺ was consequently displaced into soil solution.Additions of amendments also increased the Olsen-extractable P levels in the order: poultry manure> filter cake> household compost> grass residues and their addition also decreased theP adsorption capacity ofsoils. Concentrations of exchangeable Ca, Mg and K, and Na in the case of poultry manure, were increased by additions of organic amendments. In a glasshouse experiment, the four organic residues were applied to soils at a rate equivalent to 20 Mg ha¯¹ with or without the addition of either lime (equivalent to 0, 5 or 10 Mg ha¯¹) or P (equivalent to 0, 10 or 50 kg ha¯¹). Lime applications to the control (unamended) treatment resulted in a marked reduction in exchangeable AI, AIT AIMono and in the proportion of AIT present as AIMono in soil solution. The addition of organic amendments increased soil pH and reduced AIT and AIMono to low concentrations regardless of whether lime was applied or not. There was no yield response in maize to applied lime in any of the amended treatments. There was a yield increase in response to applied P in the control, household compost and grass residue treatments but none for the filter cake and poultry manure treatments. In agreement with this, Olsen-extractable P values in soils followed the order: poultry manure> filter cake> household compost> grass residues > control. It was concluded that the addition of organic amendments to acid soils is a practicable way of liming them and reducing the potential for Al toxicity and that it can also reduce fertilizer P requirements. This research now needs to be extended into the field situation.
The effects of surface-applied poultry manure on top- and subsoil acidity and selected soil fertility characteristics.
(2001) Judge, Angus.; Haynes, Richard John.
The effects of surface applications of poultry manure on pH, exchangeable AI, exchangeable Al saturation and levels of available macronutrients in the surface and subsurface layers were investigated in some acid soils from the KwaZulu-Natal Midlands. Three field sites with a history of long-term applications of poultry manure were compared with adjacent fields where no manure had been applied. Results generally showed an increase in pHwater, pHKCI, exchangeable Ca, Mg, and K and extractable P and a decrease in exchangeable Al and exchangeable Al saturation in the surface soil to the depth to which the manure had been incorporated. Results also provided evidence for substantial downward movement of Ca and Mg into the subsoil layers (i.e. 30-60 cm) and their accumulation in exchangeable forms. There was a concomitant general increase in pHKcl and pHwater and decrease in exchangeable Al and exchangeable AI saturation in the subsoil layers. These results demonstrate that, under field conditions, surface applications of poultry manure can cause the amelioration of subsoil acidity. This is an extremely important finding since subsoil acidity is characteristically extremely difficult and costly to ameliorate. A leaching column study was subsequently conducted to investigate the mechanisms by which surface applications of poultry manure ameliorate both top- and subsoil acidity. The soil used, originating from one of the field sites, had not previously been treated with poultry manure and had a subsoil pHKCI of 4.25 and an exchangeable acidity concentration of 1.79 cmolJkg. Three forms of poultry manure (layer, broiler and free range) were incorporated into the surface 5 cm of soil columns at rates equivalent to 5, 10 and 30 Mg/ha. Columns were maintained in glasshouse conditions for a period of 108 days and over that period they were leached on four separate occasions (receiving a total of 825 mm of simulated rainfall). At the conclusion of the experiment, the soil columns were cut into sections for chemical analysis. Applications of poultry manure to the surface soil markedly increased pHwater, pHKcl, exchangeable Ca, Mg, K and Na concentrations and decreased exchangeable Al levels in the surface 5 cm layer. They also increased the concentrations of soluble C, soluble salts, total Al and organically-complexed Al in soil solution. These effects were most pronounced with layer manure and greater at the higher rate of application. The manure-induced decrease in exchangeable AI, but increase in total AI, in soil solution was attributed to soluble salts, originating from the manure, displacing exchangeable Al into solution where it was subsequently complexed by soluble organic matter. Analysis of subsoil layers (5-15, 15-25, 25-35 and 35-45 cm) at the conclusion of the experiment showed that surface applications of poultry manure decreased concentrations of exchangeable Al in the subsoil but had no effect on pHKCl and depressed values for pHwater. It was suggested that manure-derived urea leached into the subsoil and was then hydrolysed causing an increase in pH and precipitation of exchangeable Al as insoluble hydroxy-AI oxides. Towards the conclusion of the experiment nitrification began to proceed, causing subsoil pHKCl values to decrease back to their original values. Analysis of the inorganic-N content of leachates and soil layers provided circumstantial evidence for this mechanism. High concentrations of soluble salts in the subsoil layers (caused by leaching from the manure) resulted in displacement of exchangeable A13+ and W into soil solution so that the electrical conductivity and concentrations of total and monomeric Al were elevated and pHwater was depressed in the subsoil (15-45 cm) of poultry manure-treated columns. It was concluded that the results underline the opposing effects that poultry manure applications have on (i) raising soil pH and lowering exchangeable Al but at the same time (ii) greatly increasing soluble salt concentrations and thus displacing At3+ and H+ back into soil solution. They also have suggested the importance of the release of N during manure decomposition in influencing soil pH (through the processes of ammonification, urea hydrolysis and nitrification) and therefore other soil chemical properties. It is, however, clear that the long-term effect of surface applications of poultry manure is generally to ameliorate subsoil acidity by raising subsoil pH and lowering exchangeable Al concentrations.
Effects of irrigation-induced salinity and sodicity on soil chemical and microbial properties and sugarcane yield.
(2001) Rietz, Diana Nicolle.; Haynes, Richard John.
The effects of irrigation-induced salinity and/or sodicity on sugarcane yield, and two growth parameters, namely stalk height and number of nodes per stalk , were investigated on a sugarcane estate in the Zimbabwean lowveld. The effects of soil salinity and/or sodicity on the size, activity and metabolic efficiency of the soil microbial community was also studied. Furrow-irrigated fields which had a gradient in soil salinity and/or sodicity which increased from the upper to lower ends of the fields were selected for this study. This gradient was recognized by decreasing sugarcane growth down from the upper to the lower ends and the appearance of salt on the soil surface at the lower ends of fields. Sugarcane growth was classified as either dead, poor, satisfactory or good; and soil samples (0-0 .15 m, 0.15-0 .3 m, 0.3-0 .6 m and 0.6-0.9 m) were taken from each of these areas. Soils from under adjacent areas of undisturbed veld were also sampled. Sugarcane growth and yields in micro-plots of the various areas of the fields were measured. Foliar samples of sugarcane were taken at 22 weeks of age and analysed for nutrient content. Soil salinity and sodicity were quantified by measuring pH(water), electrical conductivity (ECe) and cation content of saturation paste extracts and the exchangeable cation content. From this information, the sodium adsorption ratio (SARe)and exchangeable sodium percentage (ESP) were also calculated. The calcareous, vertic soils in the study area under undisturbed veld were found to have high pH values (8 to 9.5), very high exchangeable Ca and Mg concentrations and there was evidence of accumulation of soluble salts in the surface 0.15 m. Under sugarcane production, irrigation induced salinity and sodicity had developed. Under poor and dead sugarcane, high values for ECe, SARe, and ESP were generally encountered in the surface 0-0 .3 m of the profile. In addition, the pH values under sugarcane were often between 9 and 10 particularly in profiles where sugarcane grew poorly or had died. As expected, pH was positively related to ESP and SARe, but negatively related to ECe. Measurements of aggregate stability by wet sieving, the Emerson dispersion test and the Loveday dispersion score all showed that soils from the study sited tended to disperse and that dispersion was most apparent where high ESP and SARe values occurred in association with elevated pH values and relatively low ECe values. These measurements confirmed observations at the sites of low infiltration rates and restricted drainage particularly on the lower ends of fields where sugarcane had died. In addition to the above measurements it was also observed that there was a rise in the watertable under furrow irrigation and that the watertable was nearest to the surface at the lower ends of the fields. In some cases the watertable was observed to be only 0.2 to 0.3 m from the surface. Thus, death of roots due to anaerobic conditions could be occurring to a greater extent at the lower ends of the fields. Another consequence of the high watertable was that these vertic soils were observed to remain in a permanently swollen state. This limits air and water movement in the soil profile as such soils need to be allowed to dry out and crack regularly so that macroporosity can be restored. Sugarcane yield, stalk height and number of nodes per stalk were not significantly related to ECe. Sugarcane yields were, however, significantly correlated with ESP and pH while stalk height and number of nodes were negatively correlated with ESP, SARe and pH. These results suggested that sodicity was a more limiting factor for sugarcane growth than salinity. Foliar analysis of leaf tissue did not reveal substantial differences in macro- or micro-nutrient content between good and poorly-growing sugarcane. It was concluded that the gradient of decreasing sugarcane growth down the furrow-irrigated fields, with crop death at the lower ends, was the result of a combination of factors. That is, the watertable had risen due to over-irrigation and it was nearer the surface at the lower ends of the fields. Due to capillary rise of salts, this resulted in sodic and sometimes saline-sodic conditions in the surface soil. These conditions could limit plant growth through ion toxicities, plant water stress and inhibition of root growth and function and physiological processes. These would be induced by the high pH and high salt, Na and HC03- concentrations in soil solution. Poor physical conditions associated with sodicity and the continually swollen state of the soils presumably limited infiltration and aeration in the surface soil, and probably restricted root growth. In addition, it is likely that the high watertable limited effective crop rooting depth to about 0.2 m at the lower ends of the fields. The net result was that sugarcane died at the lower ends. A negative effect of soil salinity and/or sodicity was also observed on the soil microbial population. Significant negative correlations were obtained with ECe SARe and ESP with microbial biomass C and microbial activity (as measured by FDA hydrolytic activity or arginine ammonification rate). The activity of enzymes involved in C (P-glucosidase), P (phosphatase) and S (arylsulfatase) mineralization and potential nitrogen mineralization (as determined by aerobic incubation) were also negatively correlated with these factors, with the exception of arylsulfatase activity and ESP. All the above mentioned microbial population measures were also positively correlated with soil organic C content, besides potential nitrogen mineralization. The metabolic quotient, which provides an indication of stress and efficiency of the microbial community, increased considerably with increasing salinity and sodicity and decreased with soil organic C. Thus, increasing salinity and/or sodicity resulted in a smaller, more stressed, less efficient microbial community, while the turnover rate and cycling of C, N, P and S also decreased. It was concluded that salt affected soil not only causes a decline in sugarcane yield through raising the concentration of soluble salts in soil solution, but also has a detrimental effect on microbial activity and on mineralization of soil organic C, N, Sand P.
An evaluation of conventional and no-tillage systems on soil physical conditions.
(2002) Nzeyimana, Innocent.; Haynes, Richard John.
The use of no-tillage (NT) system has increased in the past few years in South Africa, but its effects on soil physical conditions have not been adequately documented. This study was undertaken to ascertain these effects, as compared to Conventional tillage (CT) system. Several sites were selected in the Bergville and Winterton areas of the midlands of KwaZulu-Natal, and at the Cedara Agricultural Research Station. NT generally increased bulk density in the topsoil and this altered total porosity and poresize distribution. Water retention, organic C and aggregate stability were increased under NT, partly due to the maintenance of the mulch cover on the surface soil. Organic C and aggregate stability were positively correlated with each other. Differences in bulk density between tillage systems with soil depth did not clearly indicate where soil compaction had occurred. Significant differences in soil compaction between treatments were, however, illustrated by changes in soil penetration resistance (SPR), especially at the. 150 mm depth. In addition, depending on the soil type, SPR was greater in the topsoil under NT than CT. It was suggested that conversion from CT to NT was carried out when the topsoil of the CT-fields was structurally poor, due to a previous history of continuous CT. Tractor traffic under CT and repeated tillage when the soil was wet had, in some cases, resulted in the formation of a compacted layer at the depth of cultivation. In clay soils, this has resulted in subsoil compaction. The formation of compacted layers caused major changes to pore size distribution and continuity and this resulted in substantially reduced hydraulic conductivity, infiltration rate,air-filled porosity and air permeability. It was concluded that compacted subsoil layers need to be broken up prior to conversion from CT to NT, and that compaction in the surface soil under NT has occurred and, in some cases, this will be a limitation to crop production. The use of minimum tillage systems should be considered and researched in these cases.
An investigation of factors contributing to soil degradation under dairy farming in the Tsitsikamma.
(2002) Milne, Ryan McKinlay.; Haynes, Richard John.; Miles, Neil.
Pasture-based dairy farming is the major land use in the Tsitsikamma region of the Eastern Cape. Permanent kikuyu grass (Pennisetum clandestinum) dominates pastures in the region. Kikuyu pastures do not, however, provide adequate year-round quality feed for dairy cows. This has led to the use of annually sown pastures with perennial ryegrass (Lolium perenne) to provide winter forage. Soil degradation under this management has, however, become recognised as a major limitation. Soil quality and degradation under annual and permanent pasture in the region were evaluated in three separate studies. These were (i) an investigation of the extent of loss of soil organic matter and related soil microbial properties and aggregate stability under annual pastures, (ii) a comparison of soil physical properties under annual and permanent pastures and (iii) a survey of the nutrient status of soils and pasture herbage in the region. In the first study, four commercial dairy farms, situated on sites which represented the three main soil groups in the region were sampled, were taken from under permanent kikuyu pastures, annual ryegrass pastures and undisturbed native vegetation nearby. In comparison with undisturbed, native vegetation, soils under both annually cultivated and permanent pasture had gained soil organic matter on the sandy, low rainfall eastern end of the Tsitsikamma. By contrast, at the higher rainfall, finer-textured, western end, where the native vegetation consists of coastal forest, there was a loss of soil organic matter under both types of pasture. Despite this, soil organic C content was lower under annual ryegrass than permanent kikuyu pasture at all the sites reflecting the degrading effect of annual cultivation on soil organic matter. As a consequence, labile, K(2)S0(4) - extractable C, microbial biomass C, basal respiration, arginine ammonification, flourescein diacetate hydrolysis rates and aggregate stability were all less under annual ryegrass than permanent kikuyu pastures at all the sites. The effects of annual ryegrass and permanent kikuyu pastures on soil physical properties and root length density were compared with those of undisturbed native vegetation on the four experimental sites. Root density and the depth of rooting were much less under annual ryegrass than under kikuyu pastures or native vegetation. There was no consistent effect of improved pastures or pasture type on bulk density and total porosity or penetrometer resistance, although annual pasture soils generally had higher bulk densities and lower total porosities than those under native vegetation. There was a tendency for smaller saturated hydraulic conductivity and air permeability under ryegrass than kikuyu pastures, regardless of whether total porosity was higher or lower under ryegrass. This was attributed to annual cultivation and subsequent natural consolidation causing a decrease in pore continuity under ryegrass pastures. Penetrometer resistance values confirmed the presence of subsoil compacted layers at two annual ryegrass pasture sites. At one such site, subsoil tillage was effective in reducing penetrometer resistance and bulk density, increasing pore continuity (as evaluated by hydraulic conductivity and air permeability) and greatly increasing root density and rooting depth. The nutrient status of soil and herbage from annual ryegrass and permanent kikuyu pastures sampled from 40 dairy farms in the Tsitsikamma region were evaluated. Along with the decreased organic matter content, there was a decrease in soil pH and a loss of exchangeable cations under annual pastures. Large concentrations of extractable P and sometimes exchangeable K were measured in soils under both ryegrass and kikuyu pastures and it was concluded that the rates of applied P, and sometimes K, were often excessive (particularly under kikuyu). Various nutritional problems were also identified. These included the need for Ca supplementation, particularly under kikuyu, due to the low herbage Ca concentrations. The low Ca : P ratio measured in annual ryegrass pastures, and more particularly in kikuyu herbage, highlighted the low Ca content of herbage and also the tendency of kikuyu grass to accumulate large concentrations of P. The large K concentrations and high K : Ca +Mg ratios identified in pasture herbage suggest the potential for animal nutritional problems such as hypomagnesaemia. It was concluded that although kikuyu is an excellent pasture in terms of dry matter production it tends to be deficient in Ca (and sometimes Na) and can contain prohibitively high K levels, which are likely to induce Mg deficiencies in grazing animals. The micronutrient concentrations in herbage were generally adequate, although copper concentrations tended to be low suggesting that fertilizer applications and/or feed supplementation is required. It was concluded that annual conventional tillage results in a substantial loss of soil organic matter, soil microbial activity and aggregate stability under annual ryegrass pastures when compared to those under permanent kikuyu grass. This loss of soil organic matter can result in natural consolidation of the soil in the cultivated layer and exasperated through treading by the grazing cows. The annual cultivation can also lead to the formation of a subsoil compacted layer. Nonetheless, compaction can also occur under permanent pasture presumably due to treading damage. Careful management to avoid treading damage to pastures should be practised. In order to protect the organic matter status of annual pastures, direct drilling of such pastures should be seriously considered. In some cases, annual fertilizer P rates (and to lesser extent those of K) could be reduced considerably since the levels accumulated in the soils are excessive.
Sorption and desorption of pyridine by Pahokee peat from hexadecane in the presence of organic co-solvents.
(2002) Reddy, Minolen Kistensamy.; Jaganyi, Deogratius.; Borisover, Mikhail.; Graber, E.
A study of the interactions of the specifically interacting organic compound pyridine with a model soil organic matter sorbent (Pahokee peat) was carried out from different nonaqueous organic liquid media, including neat n-hexadecane, acetonitrile, acetone and nhexadecane mixtures with either acetone or acetonitrile. Kinetic and equilibrium studies using an activity-based comparison of the organic compounds in solution was used to study the interactions of soil organic matter (SOM) and pyridine sorption capability in the various non-aqueous organic liquid media. Quantification and qualification of pyridine and the other co-solvents were done using Gas Chromatography (GC). Sorption of pyridine from neat organic solvents was not masked by sorption of the organic solvent. The apparent sorbed amount calculated from the change in solute concentration and reported on a dry weight basis was considered to represent the true sorbed concentration of pyridine in the sorbent phase. Pyridine sorption was found to be non-linear and distribution coefficients decreased with solute concentration, by approximately three times in n-hexadecane, more than five times in acetonitrile, and by ten times in acetone over the experimental concentration range. Pyridine sorption from nhexadecane was also found to be comparable with sorbed amounts from acetone, but much lower in comparison to sorption from acetonitrile. Sorption of pyridine from n-hexadecane mixtures with acetonitrile or acetone demonstrated the solvent assisted effect of pyridine sorption. Sorption uptake of pyridine increased as initial acetonitrile concentration increased, this acetonitrile assisted trend for pyridine sorption was found in the presence of a large excess of n-hexadecane. Sorbed concentrations of pyridine measured in the presence of high concentrations of acetonitrile (close to it's solubility limit) were found to be very similar to pyridine sorption from neat acetonitrile. Sorption behaviour of pyridine in n-hexadecane-acetone mixtures showed that increasing acetone concentrations had no effect on pyridine sorption. Pyridine sorbed from n-hexadecane, n-hexadecane-acetonitrile, and n-hexadecaneacetone mixtures showed a hysteretic desorption to n-hexadecane. After a series of repeated solvent extractions with solvents of increasing solvating power(1,4-dioxane, ethanol, dimethylsulfoxide), a fraction of pyridine remained bound to the peat. This nonrecoverable fraction was approximately the same for the different organic media (OA5± 0.09 in n-hexadecane suspensions, 0.57±O.12 in n-hexadecane-acetonitrile mixtures, and OA6±0.07 in n-hexadecane-acetone mixtures). Acetonitrile sorption by peat from nhexadecane was found to be very non-linear and hysteretic. The acetonitrile sorbed was almost fully recoverable, around 90%, for the initial acetonitrile concentration range varying from 0.14-0.7% by volume. However in the presence of pyridine a significant portion of acetonitrile was not recovered even after multiple extractions of polar organic solvents. Pyridine irreversible binding was not induced by acetonitrile additions and was found to occur to the same extent in both neat n-hexadecane and n-hexadecane-acetone mixtures. The solubilities of acetonitrile and acetone were determined by the flask method at 25°C using GC analysis. Solubility in volume percent for acetonitrile in n-hexadecane, 0.9±0.07, 0.57±0.02 for n-hexadecane in acetonitrile, 24.0±OA for acetone in nhexadecane, and 13 A±O.2 for n-hexadecane in acetone, were found. Log Ostwald coefficient (1.63±O.02) for acetonitrile in n-hexadecane was measured at 25°C using head space analysis and was found to be constant in the acetonitrile concentration range 0.10.8% by volume. Log Ostwald coefficient for pyridine in hexadecane used was 3.02, for the pyridine concentration range 50 mgIL-500 mg/L, this value was constant even with 0.5% by volume additions of acetonitrile. Analyses of sorption isotherms were reported on an activity basis to eliminate the effect of differential solute interactions in the solvent, calculated using the solute equilibrium concentration, the concentration of saturated vapour, and the Ostwald coefficient. Dissolution of peat components into n-hexadecane are known to be negligible. Peat components extracted after 12 hours and 3,5 months acetonitrile and acetone treatment (solid liquid ratio 1: 10) Showed 15 to 20 times less visible absorbance respectively (A. 465, 620, and 665, E4:E6 ratios using DV-Visible Spectroscopy), than the 12 hours aqueous peat extract. Quantification of the dissolved humic materials in the aqueous extract was followed using a Total Organic Carbon analyser. The study found the degree of humification to be much lower in non-aqueous organic solvent extracts (2.5 for acetone extracts, and 3 for acetonitrile extracts) than in aqueous solution extracts (8.2).
Comparative effects of sugarcane monoculture on soil organic matter status and soil biological activity.
(2002) Dominy, Carol Susan.; Haynes, Richard John.
The effects of increasing periods under sugarcane monoculture (managed by preharvest burning) on soil organic matter content and related soil properties were investigated in the 0 to 10 cm layer of a sandy coastal Ochric Cambisol (Glenrosa soils) and a red Rhodic Ferralsol (Hutton soil) from the sugar belt of KwaZulu-Natal. The organic C content at both sites under undisturbed vegetation is about 48 g C kg-I. This declined exponentially with increasing years under sugarcane. For the Glenrosa site, organic C reached a new equilibrium level of about 20 g kg-I while at the Hutton soil the equivalent value was 41 g kg-I. The higher organic matter content maintained at the Hutton site was attributed mainly to clay protection of organic matter since the clay content of the Hutton soil was 61 % compared to the 18 % for the Glenrosa soil. The loss of soil organic matter under sugarcane resulted in a concomitant decline in soil microbial biomass C, microbial quotient, basal respiration, aggregate stability, arylsulphatase and acid phosphatase activity. The activities of arylsulphatase and acid phosphatase like those for concentrations of microbial biomass and organic C, were higher for the Hutton than Glenrosa soils. At the Glenrosa site, the natural OBC abundance in soils was used to calculate the loss offorest-derived, native soil C and the concomitant input of sugarcane-derived C. Sugarcane-derived C increased over time until it accounted for about 61 % of organic C in the surface 10 cm in soils that had been under sugarcane for greater than 50 years. The effects of agricultural land use (including burnt sugarcane) on organic matter content and related soil properties were compared with those under undisturbed native grassland in KwaZulu-Natal. Two separate farms situated on Oxisols were used and both contained fields with continuous long-term (>20 years) cropping histories. At site 1, soil organic C content in the surface 10 cm followed the order permanent kikuyu pasture> annual ryegrass pasture> native grassland> preharvest burnt sugarcane > maize under conventional tillage (CT). At site 2, organic C in the surface 20 cm decreased in the general order kikuyu pasture> native grassland > annual ryegrass pasture> maize under zero tillage (ZT) ~ maize (CT). Organic C, microbial biomass C, microbial quotient, basal respiration and aggregate stability were substantially greater in the surface 5 cm under maize ZT than maize CT. In the undisturbed sites (eg native grassland and kikuyu pasture) the metabolic quotient increased with depth. By contrast under maize CT and sugarcane there was no significant stratification of organic C, yet there was a sharp decrease in metabolic quotient with depth. Aggregate stability was high under both native grassland and kikuyu pasture and it remained high to 40 cm depth under the deep-rooted kikuyu pasture. Although soil organic C was similar under maize CT and sugarcane, values for microbial biomass C, microbial quotient, basal respiration and aggregate stability were lower, and those for metabolic quotient and bulk density were higher, under sugarcane. This was attributed to the fallow nature of the soil in the interrows of sugarcane fields. It was concluded that the loss of soil organic matter, microbial activity and aggregate stability is potentially problematic under maize CT and sugarcane and measures to improve organic matter status should be considered. For sugarcane, this could include green cane harvesting and the use of green manure crops in rotation.
Influence of land use on soil organic matter status, microbial biomass C and size and composition of earthworm communities in northern KwaZulu-Natal.
(2002) Dlamini, Thembisile Charity.; Haynes, Richard John.
The effect of land management including undisturbed native forest, native grassland, sugarcane (preharvest burnt or green cane harvested), exotic forest (gum, pine or wattle), orchard crops (banana, orange and avocado) and grazed kikuyu grass pastures on soil organic matter status, size of the microbial biomass and size and composition of the earthworm community was studied. The study locality was in the tropical, northern part of KwaZulu-Natal near the town of Eshowe and sites were on a number of sugar estates in the area. Concentrations of soil organic C were notably high under kikuyu pasture, native forest and banana and lowest under burnt cane. Among the land uses, values followed the order: kikuyu pasture ≥ native forest > banana > native grassland = orange ≥ trashed cane = gum ≥ pine ≥ avocado > wattle ≥ burnt cane. Soluble C was considerably higher under kikuyu pasture than other land uses. Soils under native forest and banana also had relatively high values while lowest values were recorded under burnt cane. Values for microbial biomass C showed broadly similar trends with land use to soluble C. Very high values for microbial biomass C (> 2000 mg kg⁻¹ ) and microbial quotient (> 4.5 %) were recorded under kikuyu pasture, native forest, banana and orange whilst lowest values for microbial biomass C ( 250 - 750 mg kg⁻¹ ) and microbial quotient (1- 2 %) were found for soils under avocado, trashed and burnt sugarcane. Earthworm numbers followed the order: kikuyu pasture > native forest > banana > orange > wattle = pine = gum = trashed sugarcane ≥ native grassland ⁻¹ avocado > burnt sugarcane. Values for earthworm numbers and biomass were closely correlated. Earthworm numbers, microbial biomass C and soluble C were closely correlated with each other but none were significantly correlated with soil organic C content. Earthworm numbers were also positively correlated with soil pH and exchangeable Ca content. A total of 11 species of earthworm were collected from the sample sites. Over 80 % of the individuals collected were accidentally-introduced exotic species which originated from India, South America and West Africa. Most land uses supported between 5 and 7 species. Wattle forest and sugarcane, however, had only 2 or 3 species. Juveniles dominated the community under all land uses except kikuyu pasture and avocado where the majority of earthworms were adults. Epigeic species dominated the community under native forest and native grassland and this was also the case under avocado and gum. For the other land uses endogeic species predominated. The most numerous earthworm species present was Pontoscolex coreththrurus which was present under all the land uses. It is a peregrine, endogeic species originating from South America and is thought to have been introduced via India. The most common epigeic species was the Indian species Amynthas rodericensis which made up a particularly notable portion of the community under native and gum forests, avocado and banana. The third most numerous species was A. minimus, also from India, which is a polyhumic, endogeic species. It was particularly numerous under kikuyu pasture. In 8 out of 11 land uses, P. corethrurus, A. rodericensis and A. minimus coexisted together. Another polyhumic, endogeic species, Dichogastersaliens, which originates from West Africa, was present particularly under oranges, wattle and sugarcane. The only land use that contained mainly native species was native grassland where Tritogenia douglasi and Acanthodrilidae sp predominated. It was concluded that organic matter content, microbial biomass C, soluble C and the size and composition of earthworm communities in soils of the study area are greatly affected by land management practice. As is the case in most other parts of the world, the earthworm community under agricultural land management is dominated by accidentally introduced exotic species and these have also emigrated into soils under native vegetation; The role of these species in influencing soil chemical, physical and microbial properties, and thus soil fertility, deserves further studying.
The characterisation of some South African water treatment residues and glasshouse pot experiments to investigate the potential of two residues for land disposal.
(2003) Titshall, Louis William.; Hughes, Jeffrey Colin.
Water treatment residues (WTRs) are the by-product from the production of potable water. They consist mainly of the precipitated hydrous oxides of the treatment chemicals, and materials removed from the raw water. This study investigated the range of treatment processes and residues produced in South Africa, and two WTRs were selected for testing on selected soils and mine materials. A questionnaire was developed and sent to water treatment authorities across South Africa. Information on the treatment chemicals, dosages, volumes and current disposal practices, and a sample of WTR from each treatment plant were requested. Eleven, of 21 authorities, returned completed questionnaires, representing 37 water treatment facilities. Organic polymers were the most commonly used treatment chemical, with most plants also using lime. Other less frequently used chemicals and additives were A12(SO4)3.14H2O, Fe2(SO4)3, FeC1), sodium aluminate, activated silica, activated charcoal, CO2 and bentonite. Information given regarding residue thickening and disposal was poor. Samples from Rand Water, Umgeni Water (Midmar), Midvaal Water Company, Amatola Water and Cape Metropolitan Council (Faure) were received or collected. An additional sample from Faure was also received, representing a change in the treatment process. These samples were analysed for a range of chemical and physical characteristics. These analyses showed that the WTRs had the potential to supply some plant nutrients (Ca, Mg, Fe, S) but that metal toxicity may be a problem, in particular Mn in the Faure WTR, and that P adsorption may be severe. The samples selected to test the potential for land disposal were from Rand Water and Faure. A pot experiment tested the growth of Eragrostis teff, Cenchrus ciliaris and Digitaria eriantha in mixtures of Rand WTR and material from a coal mine i.e., a sandy soil material, spoil material and coal combustion ash, at rates of 0, 50, 100, 200 and 400 g kg-1 with a uniform fertiliser treatment applied to all mixtures. The grass was harvested on three occasions and the mean total yield (dry mass) determined, as well as nutrient uptake. The pots were leached after each harvest and the pH and electrical conductivity determined. The soil, spoil and ash were characterised and pH, EC and water retention characteristics of the mixtures determined. Growth of the grasses in the ash treatments was poor and these were terminated. Eragrostis teff grown in the soil showed a decrease in mean total yield with increasing WTR application rate, but yield was good up to the 200 g kg-1 treatment at the first harvest, declining substantially by the second harvest. In general C. ciliaris and D. eriantha grown in the soil showed a decrease in mean total yield for all harvests with increasing WTR application. The yield of E. teff, grown in the spoil, increased up to 100 g kg-1 WTR addition, but decreased thereafter. Digitaria eriantha showed a decrease in yield, and C.ciliaris an increase, with increasing WTR application rate, but for all treatments the differences were non-significant. The pH and EC of the leachates generally increased with increasing WTR addition. The concentration of nutrients in the grasses did not indicate any deficiencies or toxicities. As the growth of grass was poor in the ash treatments, another pot experiment was established to test the growth of two creeping grass species grown in the Rand WTR as a cover over the ash material. Cynodon dactylon and Stenotaphrum secundatum were grown in 20, 40 and 60 mm layers of Rand WTR, with and without a fertiliser treatment. Both species performed best in the 60 mm layer with fertiliser, and C. dactylon performed better than S. secundatum. The former species was more tolerant of the high pH, but both have potential as cover vegetation on the ash dumps when these are covered with Rand WTR. A further glasshouse study investigated the effect of Faure WTR mixed with a nutrient poor sandy soil on the nutrient uptake and seed yield of common dry beans (Phaseolus vulgaris). The WTR was added to the soil at 0, 50, 100, 200 and 400 g kg-1 each with five levels of fertiliser (0, 25, 50, 100 (recommended optimum) and 150 %). Bean pods were harvested once the plants had senesced. The number of pods and mass and number of seeds per treatment were determined. The seeds were analysed for nutrient uptake. Interveinal chlorosis and necrotic lesions were evident on cotylendonous and new leaves in the WTR treated soils, the severity of the symptoms increasing with increasing rate of WTR. Additional pots were established at the 400 g kg-1 rate (without fertiliser) and leaf material collected for chemical analysis. This showed that Mn toxicity was the cause, with leaf concentrations about 12 times the recommended 100 mg kg-1 upper limit. However, mass of bean seed was highest in the 400 g kg-1 Faure WTR treatment with 150 % fertiliser. Nutrient translocation to the seed seemed to be relatively consistent regardless of treatment, with little accumulation of Mn. The data collected illustrated the range of conditions and types of WTRs produced in South Africa, and that in some instances these residues have favourable characteristics for land application. The use of the Rand WTR showed that it could be applied to the spoil medium at relatively high concentrations without severely negatively impacting on grass growth, but that more caution should be used when applying this material to the soil medium. While the grass did not grow in the ash treatments, it would seem that with suitable species the Rand WTR could be beneficially applied to ash material as a cover layer. The use of the Faure WTR on a sandy soil seemed to potentially improve the yield of the indicator crop, but caution should be exercised due to the possibility of Mn toxicity. The use of additional fertiliser would seem to be essential. Further research would require that field scale investigation of both WTRs be conducted, as well as further studies of application rates and techniques in laboratory and glasshouse investigations.
In vitro polyploidization of selected indigenous plant species.
(2005) Reddy, Viloshanie.; Smith, Michael Trevor.
Many plant species indigenous to South Africa have ornamental, medicinal and horticultural value. Polyploidization is one technique that has been used to artificially produce superior genotypes, particularly in horticultural species. In the current investigation two antimitotic substances, colchicine at concentrations of 0.1% and 0.01% and oryzalin at concentrations of 0.01% and 0.001%, were used in an attempt to polyploidize microshoots of Dorotheanthus bellidifonnis (Burm.f.) N.E.Br and Mondia whiteii (Hook.f.) in vitro. Microshoots of D. bellidifonnis and M. whiteii obtained from nodal cuttings of in vitro germinated seedlings were maintained for 48 hours in liquid medium containing the antimitotic substances and thereafter cultured on sucrose-supplemented MS medium. The treated microshoots were evaluated for elongation, necrosis, contamination and phenolic exudation. Best results were observed in M. whiteii microshoots treated with antimitotic substances and transferred on to solid sucrose-supplemented MS medium containing 0.2% activated charcoal for 4 weeks. Leaves from the surviving treated plants were excised and used for flow cytometric analyses to evaluate changes in chromosome number. Shoots of M. whiteii treated with 0.01 % colchicine showed no changes in chromosome number, while the higher concentration used produced polyploids and mixaploids. However, oryzalin at 0.01 % concentrations produced a comparatively higher number of microshoots that were polyploids and mixaploids. Shoots of M. whiteii that have altered chromosome number have been transferred onto multiplication medium, for future evaluation of changes in phenotypic characteristics. The germination response of seeds of D. bellidifonnis was evaluated in the presence of oryzalin (0.01% and 0.001%) and colchicine (0.1% and 0.01%). Poor germination was observed in seeds germinated in the presence of 0.01 % oryzalin. Upon transfer of the germinated seedlings treated with antimitotic substances onto sucrose-supplemented MS medium, subsequent growth and development was restricted. Shoot and root development was different for the seedlings germinated in the presence of the two antimitotic substances. Shoot elongation and root development was vigorous in seedlings geminated in the presence of 0.01 colchicine and stunted development was observed in seedlings germinated in 0.1 % colchicine. On the other hand restricted rootind was observed in seedlings germinated in the presence of oryzalin, and the shoots lacked pigmentation. Meristematic cells excised from the shoot tips of the treated seedlings showed several ultrastructural changes including abnormal mitochondrial development, endomembrane formation and vacuolation. It was concluded that oryzalin and colchicine influence ultrastructure in plant cells differently. Practical constraints associated with plant tissue culture also influence the rate of in vitro polyploidization. Since different plant species require different conditions for optimal growth, it was also noted that no unique polyploidization treatment can be used for a wide range of plant species, individual species require different growth conditions.
Phosphorous dynamics in soils under contrasting long-term agricultural management practices in the KwaZulu-Natal midlands.
(2006) Majaule, Ugele.; Haynes, Richard John.
Little is known regarding the effects of land use on soil organic matter and P status of South African soils. For that reason, the effects of the main agricultural land uses in the midlands region of KwaZulu-Natal [maize (Zea mays), sugarcane (Saccharum spp), annual ryegrass pasture (Lolium multiflorum), permanent kikuyu pasture (Pennisetum clandestnum), gum (Eucalyptus grandis) and pine (Pinus patula)] on soil organic matter content, microbial biomass C and P and inorganic and organic P pools derived from a modified Hedley P fractionation was investigated on two sites where the longterm history of land management was known. In comparison with undisturbed native grassland, permanent kikuyu pasture resulted in an increase in organic C, organic P and microbial biomass C and P. Maize and sugarcane production resulted in a decrease in organic C, organic P and microbial C and P. Under annual pasture, gum and pine forests, organic matter and microbial biomass concentrations remained similar to those under native grassland. Under native grassland, extractable organic P accounted for 50% or more of the total P content of soils but under agricultural management with regular applications of fertilizer P, there was an increase in the percentage of total P present as inorganic P. Agricultural management greatly affected the distribution of P among the various inorganic and organic P fractions. Resin-Pi and NaHC03-Pi (the potentially-available forms of Pi) showed similar trends with land use being greatly elevated under kikuyu pasture at both sites and sugarcane and maize at one site. This accumulated Pi was thought to have originated from recent fertilizer applications and possibly recently mineralized organic P. Trends for NaOH-Pi with land use differed greatly from those of the Resin- and NaHC03Pi fractions. Concentrations were notably high under maize and sugarcane production. Of the pools of soil organic P, the NaHC03-Po fraction was most greatly affected by land use, being elevated under kikuyu and decreased under maize and sugarcane. This supports the assertion that it is the NaHC03-Po fraction that is the most labile soil organic P pool. It was concluded that land use greatly affects soil organic C and P status, soil microbial biomass C and P contents, soil inorganic P concentrations and the distribution of P among the various P fractions. A short-term (8 weeks) laboratory incubation experiment was carried out to compare the effects of inorganic (KH2P04) and organic (cattle manure, poultry manure and maize crop residues) sources of P, applied at a rate equivalent to 30 kg P ha-1 , on soil inorganic and organic P fractions and the potential availability of soil P. Additional treatments consisted of lime [Ca(OHhl at 5 ton ha-1 and lime plus inorganic P. Applications of lime raised soil pH to a similar extent after 1, 4 and 8 weeks incubation. After 8 weeks, a small increase in soil pH was also noted for the cattle and poultry manure and maize residue treatments. For the inorganic P fractions, substantial treatment effects were observed only for the Resin-Pi fraction. The inorganic P source was more effective than the organic ones at increasing Resin-Pi after 1 and 4 weeks incubation and of the organic sources, cattle and poultry manure were more effective than maize residues. Resin-Pi concentrations generally increased between 1 and 4 weeks incubation but then declined rapidly between 4 and 8 weeks incubation. After 8 weeks incubation, treatment effects on Resin-Pi were small. Concentrations of NaHC03-Pi, dilute HCI-Pi and concentrated HCI-Pi all declined over the incubation period. There was no clear trend with incubation for NaOH-Pi although for the poultry manure and maize treatments, concentrations declined between 4 and 8 weeks incubation. In general, concentrations of NaHC03-Po were greater for organic than inorganic P sources after 8 weeks incubation suggesting microbial immobilization of P in these treatments. There were increases in NaHC03-Po and concentrated HCI-Po over the incubation period suggesting progressive immobilization of P from the Pi fractions that declined in concentration during the incubation. Concentrations of NaOH-Po were not greatly affected by incubation period. The lime treatments, however, had lower NaOH-Po concentrations than the others suggesting that liming may have stimulated microbial mineralization of Po. Residual-P concentrations increased over the incubation period. This was attributed to conversion of extractable Pi fractions into recalcitrant, non-extractable Pi forms and/or immobilization of Pinto intransigent organic forms.
A comparison of soil and foliar-applied silicon on nutrient availability and plant growth and soil-applied silicon on phosphorus availability.
(2006) Matlou, Mmakgabo Cordelia.; Haynes, Richard John.
A greenhouse study was carried out to investigate the effectiveness of soil-applied silicon (Si) with that of foliar applications for sorghum growth. Silicon sources were soil-applied as calmasil (calcium silicate) at two rates (4 and 8t/ha) and foliar applied Si including pure K-silicate, K-humate and K-fulvate (all three foliar treatments at rates of 300 and 600 ppm). Another treatment included soil applied calmasil plus low rate of foliar applied K-humate. The soils used for the greenhouse trial were Cartref, Glenrosa, Nomanci and Fernwood. Results indicated that application of calcium silicate to the soil before planting increased sorghum yield and Si uptake in three of the four soils. Silicon uptake from different experimental treatments followed the order: Calmasil 8t/ha > calmasil 4t/ha ~ calmasil + 300 ppm K-humate> K-humate = K-fulvate = pure-K silicate = control. Foliar sprays were ineffective at increasing yield, Si content of the plant tissues or Si uptake. The concentrations of exchangeable Ca, Mg as well as soil pH were significantly increased by calmasil treatments. Extractable AI concentrations were also reduced due to the Iiming effect of calcium silicate and also possibly formation of insoluble aluminosilicates. The yield response to applied calmasil seemed to be primarily related to its Iiming effect and reductions in extractable AI in the Cartref, Glenrosa and Nomanci soils. The dry matter yield was highest in Fernwood and lowest in Cartref soil. However, there was no significant yield response to calmasil in Fernwood soil which had an initial pH of 5.8 and insignificant extractable AI concentrations. Therefore application of calcium silicate had no significant effect on extractable AI concentration in this soil. Yield response to calmasil may also have been partly due to direct positive effects of applied Si on crop growth through mechanisms such as increased photosynthetic rate and reduced transpiration rate, Addition of calmasil increased the concentrations of Si in the plant tissues and reduced those of N, P and Kin Nomanci and Fernwood soils respectively. This indicates that nutrient interactions were occurring in the plant. It was concluded that foliar-application is not an effective way of applying Si to a Siresponsive crop such as sorghum when growing in soils low in extractable soil Si. This is because Si is accumulated in plant tissues in similar amounts to macronutrients. It was also concluded that in future, studies of crop response to applied Si should include the use of non-Iiming source of Si (e.g. silicic acid) so as to separate a liming effect of calcium silicate from effect of applied Si. In a laboratory study, the effects of applied silicic acid, calcium silicate and calcium hydroxide on levels of extractable P in two Si-deficient soils were investigated. Two soils (Fernwood and Nomanci soils) were treated with two rates of P and three soil amendments (calcium silicate, calcium hydroxide and silicic acid) and incubated for six weeks at room temperature. Phosphorus was extracted using Truog, AMBIC and resin methods, and levels of exchangeable and solution AI and extractable and solution Si were also measured. Application of calcium silicate and calcium hydroxide increased soil pH in both soils while silicic acid additions had no significant effect compared with the control. The pH increase was much greater in the Fernwood than Nomanci soil because of the low buffering capacity of the sandy Fernwood soil. Exchangeable AI and concentrations of monomeric and total AI in soil solution generally followed the order: control ~ silicic acid> calcium silicate> calcium hydroxide. The lowering of soluble AI concentrations in the silicic acid treatments was attributed to formation of insoluble aluminosilicate compounds while that in the calcium silicate and calcium hydroxide treatments was attributed to their Iiming effects causing a rise in pH. Concentrations of Si in soil solution were lower in the calcium hydroxide than the control treatment suggesting the solubility of Si decreased with increased pH. Additions of both Si sources increased Si concentrations in solution and the effect was more marked for the calcium silicate treatment. This was attributed to formation of insoluble aluminosilicates in the silicic acid treatment. Concentrations of H2S04extractable Si with treatment did not closely follow the same trends as those for Si concentrations in soil solution. That is, levels of extractable Si were very much higher in the calcium silicate than silicic acid treatment in both soils. In addition, concentrations of extractable Si in the calcium hydroxide treatment were similar to control in the Nomanci soil, while for the Fernwood soil, concentrations in the calcium hydroxide treatment were exceptionally high. It was suggested that liming with calcium silicate or calcium hydroxide had rendered some Si-containing compounds in the soil acid-extractable and that the nature of acid-extractable Si fraction need further study in future. The quantities of P extracted from the two soils by the various extractants followed the order: Truog> AMBle> resin. The greatest increase in extractable P induced by additions of P was recorded for Truog P and the least for resin P. The effects of Iiming (addition of calcium silicate or calcium hydroxide) on extractable P levels differed depending on the soil and extractant used with increase, decrease or no effect being recorded. Such results confirm the complexity of lime and P interactions which occur in acid soils. Additions of silicic acid had no effect on levels of extractable P, compared to control. It was suggested that the reason for this was that phosphate is adsorbed to AI and Fe oxide surfaces much more strongly than silicate. As a result, additions of Si are ineffective at increasing extractable P levels.
The potential for groundwater contamination arising from a lead/zinc mine tailings impoundment.
(2006) Vergunst, Thomas Maarten.; Bester, H. Christopher.
The mining industry produces vast quantities of overburden and mill tailings. In many instances the disposal of these wastes on the Earth's surface have caused local, and occasionally even regional, water resources to become contaminated. Contamination typically arises from the oxidation of metal sulfide minerals contained within these wastes. Upon oxidation these minerals release sulfate, their associated metal cations and acidity into solution. This study investigated the potential for groundwater contamination arising from a Pb/Zn tailings impoundment in the North West Province of South Africa (Pering Mine). The tailings is composed predominantly of dolomite, which imparts to the material an alkaline pH and a high acid buffering capacity. Acid-base accounting (ABA) established that the capacity of the tailings to buffer acidity surpasses any acid producing potential that could arise from pyrite (FeS2), galena (PbS) and sphalerite (ZnS) oxidation. These minerals account for about 3 to 6% of the tailings by mass. Total elemental analysis (XRF) showed that the material has high total concentrations of Fe (19083 mg kg-I), Zn (5481 mg kg-I), Pb (398 mg kg-I), S (15400 mg kg-I), Al (9152 mg kg-I) and Mn (29102 mg kg-I). Only a very small fraction of this, however, was soluble under saturated conditions. An estimation of potentially available concentrations, using the DTPA extraction method, indicated that high concentrations of Zn (1056 mg kg-I), and moderate concentrations of Pb (27.3 mg kg-I) and Cu (6.01 mg kg-I) could potentially be available to cause contamination. A number of leaching experiments were undertaken to accurately quantify the release of elements from the tailings material. These experiments were aimed at determining the potential for groundwater contamination and also provided a means whereby the long-term release of contaminants could be modelled using the convection-dispersion equation for solute transport. Four leaching treatments were investigated. Two consisted of using distilled water under intermittent and continuous flow, while a third used intermittent flow of deoxygenated distilled water to assess leaching under conditions of reduced oxygen. The.mobilisation of potential contaminants under a worst case scenario was assessed by means of leaching with an acetic acid solution at pH 2.88 (after the US Environmental Protection Agency's toxicity characteristic leaching procedure). The acid buffering potential of the tailings was considerable. Even after 8 months of weekly leaching with 1 pore volume of acetic acid solution the pH of the effluent was maintained above pH 5.90. The protracted acidity caused very high concentrations of Pb, Zn, Mu, Ca, Mg, Hg and S to be released into solution. Leaching the tailings with distilled water also caused the effluent to have noticeable traces of contamination, most importantly from S, Mg, Mu and Zn. In many instances concentrations significantly exceeded guideline values for South African drinking water. Modelling solute transport with the convectiondispersion equation predicted that sol- and Mu contamination could persist for a very long period of time. (±700 years under continuous saturated leaching), while Mg and Zn concentrations would most likely exceed recommended limits for a much shorter period of time (±300 years under the same conditions). In light of the various column leaching experiments it was concluded that seepage from the Pering tailings impoundment could cause groundwater contamination. A drill-rig and coring system were used to collect both tailings and pore-water samples from eight boreholes spread out across the tailings impoundment. These investigations showed that most of the impoundment was aerobic (Eh ranged from +323 to +454 mY) and alkaline (pH 8.0 to 9.5). This chemical environment favours sulfide oxidation and as a consequence high concentrations of S have been released into the pore-water of the impoundment (S concentrations ranged from 211 to 1221 mg r l ). The acidity released as a by-product of sulfide oxidation was being buffered by dolomite dissolution, which in turn was releasing high concentrations of Mg (175 to 917 mg r l ) and Ca (62.6 to 247 mg r l ) into solution. Metal concentrations in the pore-water were low as a result of the strong metal sorbing capacity of the tailings and possible secondary precipitation. The only metal which significantly exceeded recommended limits throughout the impoundment was Hg (concentrations were between 100 and 6000 times the recommended limit of 0.001 mg r l ). Under the current geochemical conditions it is expected that Hg, S and Mg will likely pose the greatest threat to groundwater. The main concerns associated with mine tailings are that of mine drainage and dust blow off..In order to eradicate the latter problem, the tailings impoundment at Pering Mine was covered with a layer of rocks. Modelling the water balance of the impoundment using the computer model HYDRUS-2D showed that the rock cladding has potentially increased the volume of drainage water seeping from the impoundment. In light of the leaching experiments and field work, which proved that water passing through the tailings became enriched with various potentially toxic elements, it is expected that the problem of groundwater contamination around Pering Mine has been further exacerbated by the rock cladding. It was therefore concluded that there would be a strong likelihood of groundwater contamination in the vicinity of the mine.
A comparison of soil extraction methods for predicting the silicon requirements for sugarcane.
(2007) Kanamugire, Andre.; Meyer, J. H.; Haynes, Richard John.
Although silicon (Si) has not yet been recognized as an essential nutrient element, its application to sugarcane (Saccharum officinarum L.) has proved to be beneficial. Since optimum crop production depends on the maintenance of adequate plant nutrients in the soil, there is a need in the South African sugar industry for a reliable index for assessing the requirement for supplemental silicon (Si) in soils, particularly in reducing the risk of Eldana saccharina stalk borer infestation in cane. The objective of this study was to assess Si availability in soils, to select a suitable Si extraction method and a critical value for determining when a response is likely. For this purpose, five acid soils (representing. some of the most important agricultural soil groups used for sugarcane production in the sugar belt) were used in October 2004, in the lAKE WILSON glasshouse of the South African Sugarcane Research Institute (SASRI) based at Mount Edgecombe. Except for the Arcadia form soil with an initial Si content of 1.2 mmol kg- I as estimated using the O.OlM H2S04 + (NH4)zS04) extractant, soils representing the other five soil forms namely Cartref, Glenrosa, Longlands and Nomanci; exhibited a sub-optimal Si content of not more than 4.0 mmol kg-I. Sorghum was used as a plant crop and sugarcane as a ratoon crop because of their Si accumulator status. Three different Si sources: calmasil, slagment and wollastonite; with respectively 9.85, 15.20, and 5.25% Si content were applied at increasing rates of 0, 3 and 6 tons ha- 1 as Si fertilizers. Silicon (Si) was extracted from untreated and treated soils by utilizing six different extractants, (1) O.OlM H2S04 + (NH4)2S04; (2) Distilled water; (3) 0.025M H2S04; (4) 0.5M CH3COOH; (5) 0.5M CH3COONH4pH 4.8; and (6) O.OlM CaCh.2H20. The amount of soil Si extracted followed the order: 0.025M H2S04 > 0.5M CH3COOH > O.OlM H2S04 + (NH4)2S04 > O.OlM CaCh.2H20 > 0.5M CH3COON~ pH 4.8 > distilled water. Soil Si extracted by 0.025M H2S04 was significantly correlated with soil exchangeable cations,. CEC, clay content, cane biomass yield, cane Si uptake and increasing rates of applied Si. Averaged over all soil forms investigated, the increases in dry biomass yield and Si uptake ranged. from 18% to 154% for sorghum; and from 23% to 85% for cane respectively. Even though the highest increases (%) in cane biomass yield and Si uptake were obtained on a Nomanci form soil with initial poor fertility status, the highest means were obtained on an Arcadia form soil with the highest Si initial content. There was no difference between different Si sources in their ability to influence cane biomass yield and Si uptake, and therefore the supply to the soils. Even though the lower and higher Si source rates were not different from each other, they increased cane yield and Si uptake, indicating that Si was undoubtedly beneficial for sugarcane. The Si critical levels for different soils as estimated by 0.025M H2S04 were 6.0 mmol kg-1 (168 mg kg-I) for Arcadia; 2.6 mmol kg-I (64 mg kg-I) for Cartrel; 2.5 mmol kg-I (64 mg kg-I) for Glenrosa; 1.6 mmol kg-I (45 mg kg-I) for Longlands; and 2.4 mmol kg-I (67 mg kg-i) for Nomanci form soils.
A laboratory and glasshouse investigation on the effect of liming with fly ash and processed stainless steel slag on two contrasting South African soils.
(2008) Ndoro, Esina Tambudzayi.; Hughes, Jeffrey Colin.
Soil acidity is a major land degradation problem that limits crop production globally. The high cost of traditional liming materials (calcitic limestone, dolomite etc.) and the vast areas of land that require liming have led to the exploratory utilisation of alkaline industrial by- products such as fly ash and stainless steel slag. The liming potential and effects of liming with fly ash (from the Duvha power station) and processed stainless steel slag (Calmasil) on two acid soils were investigated in this study. The quality of fly ash and Calmasil as liming materials and their potential impacts on the soil quality and plant growth were investigated. The effects of liming with these materials on soil pH, EC, extractable Al, Mn, base cations and trace elements were investigated in an incubation experiment. A glasshouse trial was conducted to assess the effects of these materials on the growth of an acid intolerant crop, perennial rye grass. The incubation and glasshouse study were of a factorial design with two acid soils (the Avalon and Inanda soils), three materials (fly ash, Calmasil and lime); and five application rates of 0, 50, 100, 200 and 400% of the recommended optimum liming rate (OLR) for the growth of perennial rye grass. Characterization of fly ash showed that the major elements (>5%) present (Si > Al > Fe) are not comparable to lime (Ca > Si > Mg) and that it has a low liming potential (calcium carbonate equivalence (CCE) of 9.6%) in comparison to lime. The chemical composition of Calmasil is comparable to lime with Ca > Si > Mg as the major elements and it has a very high liming potential (CCE = 97%). The incubation experiment showed that adding fly ash and Calmasil increased the pH of both soils. However, at the optimum liming rate (100% OLR), only the treatment with Calmasil in the Avalon soil attained pH levels within the desired pH range. Extractable Al and Mn decreased with addition of fly ash and Calmasil to levels comparable to lime in the incubated soils. Addition of fly ash and Calmasil also increased the extractable base cations of both soils. The yield-response of perennial rye grass to treatments in both soils was in the following order: fly ash > Calmasil > lime. Application of fly ash at > 200% OLR in the Avalon soil caused injury of ryegrass. Application of fly ash and Calmasil at lower rates has great agronomic potential in ameliorating soil acidity.
The influence of soil properties on the vegetation dynamics of Hluhluwe iMfolozi Park, KwaZulu-Natal.
(2009) Harrison, Rowena Louise.; Hughes, Jeffrey Colin.
The physical and chemical properties of soils can greatly influence the vegetation patterns in a landscape. This is especially so through the effect that particular characteristics of soils have on the water balance and nutrient cycling in savanna ecosystems. Areas in the savanna environment found in Hluhluwe iMfolozi Park have experienced a number of changes in the vegetation patterns observed. This study, therefore, looks at the effect that soil characteristics may have on the vegetation growth in this area and on the changes that have taken place over time. Fixed-point photographs, taken every four years, were used to choose fourteen sites in the Park, which showed either a ‘change’ or ‘no-change’ in vegetation from 1974 to 1997. The sites consisted of four which had ‘no-change’ in vegetation, two sites with a slight increase (5- 20%) in tree density, three sites with a greater increase in tree density (>20%), two sites with a slight decrease in tree density (5-20%), and three sites with a greater decrease in tree density (>20%). Transects were then carried out at each site, in which the soil was classified to the form and family level. Each horizon was then sampled and the field texture, structure, Munsell colour and depth of each horizon and profile recorded. The data recorded in the field were statistically analysed through a principal component analysis (PCA). The type of horizon, horizon boundary, structure type, colour group and depth for the top and subsoil were included in the models and were analysed with the number given to each site for each of the three sections of the Park, namely Hluhluwe, the Corridor and iMfolozi. The most prominent textures at all sites were sandy loam, loam, clay loam and silt loam for both the top and subsoil for all site categories. The texture classes were also compared across the Hluhluwe, Corridor and iMfolozi sections. The dominant textures in the Hluhluwe and Corridor sections are loam, clay loam and silt loam for both top and subsoils. Sites sampled in the iMfolozi section appear to have textures mainly associated with the clay loam and sandy loam classes. The structure classes of the soil including sub-angular blocky, granular and crumb which are associated with a moderate structure appear to be the most dominant type in all categories for the topsoil; single-grain and sub-angular blocky classes the main types for the subsoil. Generally the colour of the soil at all the sites sampled was yellower than 2.5YR and the values and chromas mostly fell within the range of 3-5 and 2-6, respectively. This is also shown in the PCA results obtained, which associate particular soil characteristics with the various sites sampled for the different vegetation change categories investigated. The samples collected were also analysed in the laboratory after being air-dried. The laboratory analysis included measurements of pH, exchangeable acidity, organic carbon, extractable phosphorus, particle size distribution and cation exchange capacity (CEC). The data recorded in the laboratory were also analysed by PCA. This was used to determine which soil properties are associated with the particular sites investigated. The pH of the soil, in all areas, fell within a wide range. The pH is influenced by the rainfall in the area and thus sites sampled in the Hluhluwe section are more acidic than those sampled in the Corridor and iMfolozi sections. The topsoils had a higher pH for all the samples and were in the range between 5 and 7. The exchangeable acidity measurements were low, although they were higher in the subsoil as opposed to the topsoil. The nutrient contents did not appear to vary greatly between the different sites in the Park. Generally extractable phosphorus, CEC and organic carbon were low across the Park. The particle size analysis showed that the clay percentage increases between the top and subsoil for all the sites sampled. The silt and various fractions of sand percentages vary across all sites and are lower than the clay percentage at all sites except the A horizon of the ‘slight increase’ sites. The ‘no-change’, and ‘increase’ sites have a higher percentage of clay as compared to the silt and sand fraction for both the A and B horizon. The ‘slight increase’ sites have a higher percentage of sand in the A and B horizon, the ‘slight decrease’ sites have a more equal percentage between the sand, silt and clay fractions in the A horizon and a greater percentage of clay in the B horizon. The ‘decrease’ sites have a greater percentage of clay and silt in the A and B horizon. While certain soil properties have a definite effect on the plant growth, no relationship between specific soil properties and vegetation changes was shown. However, it is likely that the soil structure and texture affect the vegetation patterns, through their influences on the water and nutrient holding capacity. With an increase in the clay percentage and more strongly structured soils, plants can access more water and nutrients and this will increase the tree density in an area. However, the recent changes in the vegetation patterns observed in the Park appear to be more associated with other environmental factors. The soil properties analysed would have generally been more constant at the sites sampled, particularly over the relatively short period of time in this study. Therefore, the changes which were recorded in the fixed-point photographs would have been enhanced by other factors experienced in the Park, including fire and the effect that grazers and browsers have on the vegetation.
Phosphorus sorption behaviour of some South African water treatment residues.
(2009) Norris, Matthew.; Titshall, Louis William.
Water treatment residues (WTRs), which are by-products from the production of potable water, are chemically benign, inorganic materials which are suitable for disposal by land application. Their high phosphorus (P) sorption capacities have, however, generated some concern in an agronomic context where P is recognised as a growth limiting plant nutrient. The extent to which labile P pools are reduced or enhanced by WTR amendments is, therefore, a central issue with respect to their disposal by land application. Therefore, the aim of this study was, through the use of empirical adsorption isotherm equations and chemical fractionation of P within the residues, to investigate the chemical processes responsible for the retention and release of P from 15 South African WTRs. Chemical characterisation revealed considerable variation in residue properties relevant to P sorption-desorption processes. pH, exchangeable Ca and organic carbon content ranged from 4.77 to 8.37, 238 to 8 980 mg kg-1 and 0.50 to 11.6 %, respectively. Dithionate, oxalate and pyrophosphate extractable Al fractions ranged from 741 to 96 375, 1 980 to 82 947 and 130 to 37 200 mg kg-1, respectively. Dithionate, oxalate and pyrophosphate extractable Fe ranged from 441 to 15 288, 3 865 to 140 569 and 230 to 90 000 mg kg-1 respectively. Therefore mechanisms of retention were hypothesised to be residue specific, being dependent on the unique chemical properties of the sorbent. Elevated Ca and amorphous Al and Fe concentrations did, nevertheless, suggest that all residues had the capacity to adsorb high amounts of P and to retain this P in forms unavailable for plant uptake. These arguments were confirmed by the sorption study where labile P was, for all residues, found to constitute a small fraction of total applied P even at high application concentrations (128 mg P L-1). Sequential P fractionation revealed that most of the inherent P (which ranged from 1 149 to 1 727 mg P kg-1) and applied P were retained in highly resistant mineral phases or fixed within the organic component. Thus P replenishment capacities were restricted even though residual P concentrations were often within adequate ranges for plant growth. Phosphorus adsorption data was described by four empirical adsorption isotherm equations in an effort to determine possible mechanisms of retention. Sorption data was, for most of the WTRs, described by the Temkin isotherm while the Freundlich and linear models fitted data for two residues each. A key finding was that the distribution coefficient (Kd) tended to increase with the quantity of P adsorbed (S) as opposed to decrease or remain constant in accordance with model assumptions. Therefore, the models could not be used for mechanistic interpretation, even though they provided excellent descriptions of the data. The direct relationship between Kd and S suggested a mechanism of retention involving the activation of sorption sites. This notion was supported by the fractionation study which showed that P addition results in the transfer of an increasing quantity of organically bound P to resistant residual forms. Model affinity parameters were strongly correlated to dithionate and pyrophosphate extractable Al and Fe which suggested that P was adsorbed primarily through ligand exchange mechanisms. The mobility of P bound to organic fractions did indicate that P was retained through weaker forces of attraction such as monodentate ligand exchange, charge neutralisation or proton transfer. Evidence to support the notion that P is immobilised through the formation of Ca phosphates was lacking. Based on P fractionation data, it was suggested that strong chemisorption mechanisms and the diffusion of P into WTR micropores were largely responsible for the minimal quantity of P desorbed by disequilibria desorption processes. A greater quantity of P was desorbed in the presence of oxalate and citrate which suggested that plants may increase bio-available pools through the release of organic ligands. Phosphorus desorbed in the presence of these ligands did, however, decline with P addition which confirmed that the affinity of the WTR surface for P increases with P application. Therefore, it was concluded that the application of P to WTRs is an uneconomical process unless sorption sites are already saturated or immobilisation processes are inhibited. In light of these findings, it was suggested that the absence of plant P deficiencies under the field application of WTRs is due primarily to inhibited sorption.
A comparative study of the BCR sequential and batch extractions for wastes and waste-amended soil.
(2010) Elephant, Dimpho Elvis.; Bester, H. Christopher.; Titshall, Louis William.
The use of standard soil tests to assess waste materials has become a common practice in waste management. However, the suitability of using standard soil tests on waste materials is questionable. Therefore, this investigation was undertaken to compare commonly used chemical extraction methods for their ability to extract elements from soil, waste, and soil-waste mixtures. This was carried out by: · Assessing the effects of extraction time on the extractability of Al,Ca, Co, Cu, Fe, Mg, Mn, Ni, Pb, S, Si, and Zn; · Assessing the comparability between single and sequential extraction. Two manganese rich wastes namely electro-winning waste (EW) and silicate-rich smelter slag (SS) and an acid soil (Inanda, Ia) were used for this study. Waste amended soils were obtained by incubating the EW and SS with Ia soil at field capacity at a rate of 20 g kg-1 and 120 g kg-1 soil respectively, and were sampled at day 0, 7, 28, 56, and 140. The effect of extraction time was assessed on the EW, SS, and Ia soil with carbonated water used in the acid rain test conducted at 16, 20, 30, and 50 hours. The equilibration time was different for different materials and elements. The concentration of Al and Zn did not change appreciably with increasing extraction time in the EW. Similar results were found for Mg, S, and Si in the SS and S in the Ia soil. This was attributed to ‘equilibrium’ being reached before 16 hours. The equilibration time of 20 hours which would release the exchangeable and specifically adsorbed elements was obtained for Co, Mg, Si, S, and Mn in the EW. The concentration of Ca decreased with extraction time in the EW and was attributed to readsorption. The concentration of Ca and Mn in the SS and Al, Fe, and Si in the Ia soil increased with extraction time and the ‘equilibrium’ was not reached even after 50 hours. This was attributed to the release of elements due to dissolution of minerals. In the comparison between Community Bureau of Reference (BCR) sequential and batch extraction, the concentrations of Ca, Co, Mg, Mn, and S were higher in batch extraction than in sequential extraction, particularly for the EW and the EW treated soils. This was possibly caused by the readsorption of released elements during water extraction. On the contrary, sequential extraction had higher concentration of Al and Fe compared to batch extraction for the EW, Ia soil, SS treated soils, and EW treated soils. These were attributed to a continued desorption of elements and dissolution of minerals due to exposed surfaces which occurs in sequential extraction. The comparison between single and sequential extraction for the BCR sequential extraction showed that hydroxylammonium chloride (HAC) applied in sequential extraction had higher concentration of Al, Co, Fe, Pb, and Zn than the single HAC extraction. This was probably due to incomplete dissolution of minerals, precipitation of amorphous minerals, and readsorption of released elements occurring for a single HAC extraction. These were minimised for hydrogen peroxide (H2O2) extraction and hence comparability between single and sequential H2O2 extraction was observed. This was probably due to the presence of complexing agent in the extracting reagent which would minimise the formation of secondary precipitates and hence, improved dissolution of minerals was obtained. Similarly, the concentrations of elements were comparable between single and sequential aqua regia extraction. These results indicated that comparability is improved between single and sequential extraction when aggressive conditions are used. The results from this investigation indicated that when chemical extraction methods are applied to wastes, the effects of the waste properties on the results of extraction need to be well understood. Consequently, when the chemical extraction methods are used in waste management scenarios certain modifications might have to be made. These modifications include the use of a high solution:solid ratio and an extracting solution which has high complexing ability
The use of scientific and indigenous knowledge in agricultural land evaluation and soil fertility studies of Ezigeni and Ogagwini villages in KwaZulu-Natal, South Africa.
(2010) Buthelezi, Nkosinomusa Nomfundo.; Hughes, Jeffrey Colin.; Modi, Albert Thembinkosi.
In the past, the indigenous knowledge of soils of small-scale farmers in South Africa has been largely ignored in scientific research. Hence the use of scientific approaches to land evaluation has often failed to improve land use in rural areas where understanding of the prescriptive scientific logic is lacking. Despite this, it is clear that local people and smallscale farmers have knowledge of their lands based on soil and land characteristics that remain largely unknown to the scientific community. It is therefore important for researchers to understand farmers’ knowledge of soil classification and management. To address this issue, a study was conducted in the uMbumbulu area of KwaZulu-Natal to investigate the use of indigenous knowledge as well as farmers’ perceptions and assessments of soil fertility. A preliminary questionnaire was designed to explore indigenous knowledge in a group interview that was conducted prior to the study. Another questionnaire was used to elicit indigenous knowledge from 59 randomly chosen homesteads representative of the population of Ezigeni and Ogagwini villages. Six homesteads were chosen for further detailed information on the cropping history, knowledge specific to the cultivated lands, detailed soil description and fertility. Soil samples were taken from these homesteads under different land uses (taro, fallow, veld and vegetable) at 0-30 and 30-60 cm depth for laboratory analysis. This was done to determine the effect of land use on soil physical and chemical properties and soil microbial activity. For scientific evaluation a general purpose free soil survey was conducted to produce land capability and suitability maps. Farmers identified ten soil types using soil morphological characteristics, mainly soil colour and texture. These soil properties were also used in the farmers’ land suitability assessment. In addition, slope position, natural vegetation and village location were used to indicate land suitability. The amount of topsoil was also used in land evaluation. However, slope position was considered the most important factor as it affects the pattern of soils and hence their suitability. Soils on the footslope were considered more suitable for crops than those found on the midslope and upslope. The yield difference observed between villages, which were higher in Ogagwini than Ezigeni, was also used as a criterion for evaluation. Farmers attributed these yield differences for various crops to the effect of soil type on productivity. In support, scientific evaluation found that Ezigeni village had a number of soils with a heavy textured, pedocutanic B horizon and hence a relatively shallow effective rooting depth. Moreover, the Ezigeni village land suitability was limited in places by poor drainage and stoniness. These limitations were rarely found for the Ogagwini village soils. Farmers had a total of six comprehensive and well defined soil fertility indicators, namely crop yield, crop appearance, natural vegetation, soil texture, soil colour and presence of mesofauna. Results showed that farmers’ fertility perceptions are more holistic than those of researchers. However, despite this, their assessment correlated with soil analysis. There was a close relationship between scientific and indigenous suitability evaluation for three commonly cultivated crops (taro, maize and dry beans). This was further substantiated by yield measurements which were significantly higher for Ogagwini as rated by both farmers and scientific evaluation as the more suitable. The significant agreements between the scientific and indigenous approaches imply that there are fundamental similarities between them. Recognizing this and subsequently integrating the two approaches will produce land use plans relevant and profitable for both small-scale farmers and scientists.

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