Animal and Poultry Science

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Development of a protocol for the molecular serotyping of the African horse sickness virus.
(2009) Groenink, Shaun Reinder.; Young, Marion Belinda.; Watson, Gregory M. F.
African horse sickness (AHS) is a viral disease with high mortality rates, vectored by the Culicoides midge and affecting members of the Equidae family. AHS is endemic to South Africa, and, as a result, affects export and international competitiveness in equine trade, and impacts significantly on the South African racehorse and performance horse industries. AHS also has devastating consequences for rural and subsistence equine ownership. The protocol developed in this dissertation has the potential to serotype and confirm the AHS virus within a few hours at significantly less cost than current methods. It will ease the financial and time constraints of studying an outbreak in real time and has the potential to solve many of the unknown factors surrounding AHS, particularly and most importantly, the role that each serotype plays in outbreaks and the form of the disease contracted by horses.
Gastrointestinal (nematode) infections in small ruminants : epidemiology, anthelmintic efficacy and the effect of wattle tannins.
(2010) Ahmed, Mawahib Alhag Ali.; Nsahlai, Ignatius Verla.
Nematode parasites have become the biggest problem for small ruminant production in South Africa due to their resistance to the commercial anthelmintics. Notable, wattle tannin has been used as an alternative strategy for control. However, the concentration and the frequencies can likely influence its effect on the parasites control. The objective of this study was to determine the degree of pasture infestation and nematode infection in sheep and goats, as well as investigate nematode resistance to the anthelmintics, and the potential of wattle tannin in nematode control. The first study dealt with the epidemiology of internal parasites. Eight Merino ewes and eight Nguni does averaged 7-18 months of age were observed for 1 year during the months of February 2008 to January 2009 at the University of KwaZulu-Natal Research Farm (Ukulinga). Egg count per gram (EPG) and coccidian oocysts per gram (OPG) were counted according to Mc Master Technique (Hansen & Perry, 1994) by magnifying parasitic eggs from monthly rectal faecal samples dissolved in saturated sodium chloride. Faecal samples also were cultured for 15 days to identify infective nematode larvae (L3) using Baermann technique. Herbage samples were collected monthly from four paddocks as well to count L3 on the pasture. Sheep live weight was also recorded monthly. Seasonal effects was significant (P<0.05) on the EPG, OPG, faecal culture L3 and pasture L3. A higher level of infection was observed in summer (wet) than in winter (dry season). Trichostrongylus spp larvae were the most prevalent larvae (26.5%) while Strongyloides, Haemonchus contortus, Nematodirus and Cooperia spp occurred in the faecal culture by percentage of 20.9%, 16%, 16% and 14.5%, respectively. For parasite resistance, Ivermectin 1% (IVM), Closantel 5% (CST) and a combination of Abamectin 0.08% and Praziquantel 1.5% (CPA) were evaluated. Twenty four sheep (12 females and 12 males) aged between 7-18 months were used for 21 days. Animals were naturally infested by gastro-intestinal parasites. EPG and faecal culture L3 were counted on day 0, 7, 14 and 21. Closantel was the most effective. Haemonchus spp. were least affected whilst Trichostrongylus spp. were the most affected by all drugs. In the third study, wattle tannins were evaluated as an alternative nematode control drug. Three experiments (Exp.) were conducted to determine the effect of tannin concentration (Exp.1 and 2) and frequency (Exp.3) on nematode parasites. In Exp.1, 0, 0.8, 1.6 and 2.4 g tannin/kg BW were drenched for three consecutive days per sheep (16 females and 8 males, aged 8-9 months) for 21 day. In Exp.2, 30 sheep (14 males and 16 females, aged 9-18 months) were randomly allocated into three tannin treatments (0, 0.8 and 1.6 g tannin/kg BW) and drenched for a day. In Exp.3, 26 sheep (11 males and 15 females aged 9-18 months) were divided into three groups of 9, 9, and 8 sheep each. These groups were drenched with 1.6 g tannins/kg BW/day; once, twice or thrice for the 3 groups respectively. For the three experiments, EPG and L3 larvae were counted in individual feacal samples. For all tannin treatments, EPG decreased (P<0.05) over time. Though the differences among tannin levels and frequencies varied (P<0.05) over time, EPG consistently decreased with increasing tannin level and frequency. Thus 1.6 and 2.4 g tannin /kg BW for 3 consecutive days had nearly similar effects on the EPG. The results of this study are rather inconclusive that weather conditions such as rainfall had a direct effect on internal parasites development. Nematode parasites at Ukulinga Research Farm were resistant to the commercial anthelmintics used. Drenching with 1.6g wattle tannin/kg BW over three successive days is enough to reduce EPG and reduce the degree of pasture contamination.
Characterisation of indigenous Zulu (Nguni) sheep for utilisation improvement and conservation.
(2010) Kunene, Nokuthula Winfred.; Nsahlai, Ignatius Verla.; Bezuidenhout, Carel Nicolaas.; Nesamvuni, A. E.
The Nguni sheep of Zululand, South Africa, are called the Zulu sheep. They are a source of food and cash for the rural farmers of KwaZulu-Natal. There is insufficient information available about the characteristics of this breed and accordingly the breed is classified as “insecure”. Documentation of characteristics of a breed is important for its utilisation, improvement or conservation. This study was undertaken to document (i) the utilization of the Zulu sheep, (ii) some morphological characteristics, (iii) establishing a cost effective body measurement recording means and (iv) the intra- and inter-population genetic variation of the breed using random amplified polymorphic DNA markers. A survey was conducted to investigate the socio-economic and cultural values of the farmers attached to livestock including the Zulu sheep. A total of 76 rural farmers were interviewed in the areas of the Mhlathuze district in northern KwaZulu-Natal. Constraints and the indigenous knowledge of the farmers on livestock production were also recorded. The results confirmed that the Zulu sheep in the rural areas are indeed used as a source of protein and cash when necessary. Farmers reported that the Zulu sheep are tolerant to ticks and able to withstand the hot and humid conditions of northern KwaZulu-Natal. Goats and cattle as well as the Zulu sheep are also used for payment of dues in the tribal courts. Even so, Zulu sheep are not used for any cultural purposes. The system of management is fairly extensive. Some farmers apply indigenous knowledge as part of management practices. For instance, they use indigenous plants as nutrient supplements and for increasing the reproduction rate of these animals. Lack of modern animal husbandry skills was declared by the farmers as one of the main challenges. A perception among the farmers was that the Government could assist in addressing this challenge. Three populations of Zulu sheep reared extensively in three localities were used for the morphometric and genetic studies. The areas were the community of KwaMthethwa (Enqutshini), University of Zululand (UNIZULU) and Makhathini Research Station. Makhathini and KwaMthethwa are 260 and 40 km, respectively, away from UNIZULU. The morphometric study was undertaken to determine the extent of phenotypic diversity between Zulu sheep populations using six morphological characteristics. Effects of some factors (location, age, sex and season) on some of these traits were estimated. Results showed that the size of the body measurements, wither height (WH), heart girth (HG), live weight (LW) and scrotal circumference (SC) were significantly different between the populations. Variation in these body measurements was influenced significantly by the location, season, the sex and the age of sheep. Mature ewes weighed up to 32 kg whereas the rams weighed up to 38 kg. The differences in LW, HG and WH between the seasons were small. The SC increased with the age of the ram up to 28 cm for mature rams. Other traits observed were the colour and the ear length of Zulu sheep. Ear size ranged from ear buds to the most common large ears (9 to 14 cm). The dominating colours observed were brown and a combination of brown and white. Live weight prediction equations were estimated employing HG, WH and SC data. The LW prediction equations showed that the regression of HG and WH produce the best estimate equations of LW; however the HG alone also showed reliable LW estimates. Scrotal circumference was more precise for estimating the LW of younger rams below 22 months of age (R2 = 0.64 – 0.78). Fifty-two Zulu sheep from the three locations were used to assess the genetic variation within the Zulu sheep breed. A total of 2744 RAPD bands were generated ranging from 0.2 to 2 kb; ~46% of these bands were polymorphic. The genetic diversity was the lowest (5.17%) within the UNIZULU population, 8.62% within the KwaMthethwa population and highest (11.04%) within the Makhathini population. The genetic diversity between all populations was estimated at 21.91 %. Phenotypic diversity was relatively similar for the UNIZULU and Makhathini populations (41.25% and 45.63%, respectively). The phenotypic diversity between the three populations was 48.26%. Genetic and phenotypic diversity was lower for Makhathini and UNIZULU populations than for the KwaMthethwa population. It was concluded that the Zulu sheep is a smaller sized breed compared to the other South African indigenous sheep breeds like the Dorper which has been reported to have some similar characteristics to the Nguni sheep. The results confirmed that the Zulu breed has the capacity to survive without dipping and supplements during the dry season. This adaptation is of value to the communities of KwaZulu-Natal. Such characteristics warrant conserving the breed to prevent genetic erosion. The phenotypic and genetic diversity between the three populations of Zulu sheep may indicate that there is an opportunity of genetic exploitation by selecting animals based on phenotypic as well as genetic characteristics. In order to promote the conservation and sustainable use of the Zulu sheep, it was recommended that an open nucleus breeding scheme from lower-tier flocks (of the farmers) for pure breeding to nucleus flocks (in Government ranches) could be appropriate. The scheme would also address the challenges of animal husbandry as well as contribute to the improvement of the livelihood of the farmers. Farmers could use a tape measure to estimate the LW of sheep when they cannot afford scales. The morphological characteristics and the genetic diversity data generated from this study could be combined into a single data base for this sheep breed. More extensive studies, using the same or some additional phenotypic characters such as reproductive performance, need to be done. Genetic characteristics of Zulu sheep using microsatellites and mitochondrial DNA should be done to complement the present study.
An evaluation of growth and symmetry in thoroughbred foals and Holstein calves.
(2008) Halgreen, Sarah.; Young, Marion Belinda.; Nsahlai, Ignatius Verla.
Holstein calves were photographed and measured over a period of 84 days to determine the feasibility of Image Analysis as a measurement tool. It was determined that the disparity between actual measurements and image analysis measurements decreased as the bone length increased, and that image analysis could be used to monitor growth successfully in large ungulates, using the length of certain bone. Image analysis was then used to evaluate growth in Holstein calves on two weaning programs (weaned early at six weeks of age and weaned later at eight weeks of age). Calves were weighed and photographed over a period of 224 days to determine the effects of weaning on skeletal symmetry. Weaning time did have an affect on the skeletal symmetry of the calves, with calves weaned earlier found to be more asymmetrical. The extent to which skeletal symmetry is affected by a stress is determined by the nature and duration of the stress. The growth and development of Thoroughbred foals was recorded and photographed for a period of 300 days and the relative asymmetry for bilateral traits was assessed. Thoroughbred foal growth correlated to statistics reported over the last twenty eight years. Asymmetry can be detected in growing foals using image analysis. Weaning stress produced some asymmetry that the foals were able to recover from. One can use highly correlated body weight and height measurements to produce a tool stud managers can used to monitor growth. In young training Thoroughbreds (between 18 and 24 months of age), no significant asymmetry was found in the forelimbs but the hind legs displayed asymmetry, which has interesting implications for training and for performance criteria in the racehorse. Tools can be produced to monitor the growth and development of Thoroughbred foals destined to race, which can improve their management and the duration of their racing careers.
Prophylactic strategies in the control of African horse sickness.
(2008) Simpkin, Tarryn Lyn.; Young, Marion Belinda.
African horse sickness (AHS) is a non-contagious viral disease transmitted by an arthropod vector and is endemic to sub-Saharan Africa. The disease affects all equine species, but is more severe in horses and other equid species not native to Africa. Vaccination is the only demonstrated means of its prevention. The horse-owning public provides much anecdotal evidence of prophylactic strategies, such as repellents, stabling, alternate hosts, traps, paraffin, blankets, smoke or fans. The present study investigated the relationship of these strategies to the incidence of AHS, and evaluated alternate hosts, wind speed and repellents on the activity of males and females of the different Culicoides species.. Cypermethrin and citronella-containing repellents repelled the most female midges. Sheep and cattle offer an alternate blood meal to gravid and nulliparous female midges. Fans are very effective in keeping midges away from horses. Methods are summarised for the horse owner to implement in addition to vaccination to prevent AHS.
Modelling broiler populations for purposes of optimisation.
(2008) Berhe, Esayas Tesfasellassie.; Gous, Robert Mervyn.
With the narrow margin of profit in the broiler enterprise, how can producers increase profit potential? It is not an easy task to answer this question since the net financial return depends on many factors; some are related to the animal, some to the feed, some to the environment and others are outside the production system, like availability and cost of labour and capital. Many researchers have attempted to improve the efficiency of the system using alternative management strategies and to develop a unified theory that could simultaneously evaluate all the relevant factors and the interactions between them. Simulation models are seen as the most promising means of moving this subject forward. Geneticists are continually improving the potential growth rate of broilers, yet there has been little change in feed specifications for these birds over the past few decades. Only recently has it been possible to make use of simulation models to optimise the feeds and feeding programs of modern broiler strains at a commercial level, but little testing of these programs has been carried out. What is needed is a thorough investigation of these models, which at present are based on an individual, as opposed to a population response. Modelling plays an increasingly important part in animal science and research as a way of organizing and evaluating the large body of existing knowledge. With the use of an accurate description of the potential growth rates of broiler genotypes, it is possible to make more efficient use of growth models which are becoming more abundant in the industry and which, in turn, enable the nutritionist or producer to predict the performance of animals when subjected to a given feed or feeding programme. The predictions made by most of the growth models now available are based on individual animals, and the results obtained may be inadequate in optimising the nutrient requirements of a broiler population because of the variation that exists in these populations. Variation in performance traits in broilers may be the result of variation in the genotype, in the environmental conditions within the house, and in the composition of the feed offered to the birds, and these sources of variation cannot all be accommodated in a model that simulates the food intake and growth of just one bird. But if variation is to be incorporated into growth models, it is necessary to ascertain the effects of variation in the various genetic parameters on the mean response of the population. A sensitivity analysis is useful in accomplishing this objective. Similarly, it is important to know what the optimum size of a simulated population should be, that takes account both of the accuracy of the simulation and the time taken to complete the exercise. This is especially important when optimisation routines are followed, as such calculations are time consuming. As a means of addressing these issues, simulation exercises were conducted using EFG Broiler Growth Model version 6 and EFG Broiler Optimiser Model version 1 (EFG Software, 2006) to determine: (a) whether it is worth generating a population when optimising feeds and feeding programs for broilers, rather than using the average individual, (b) the size of the population required to obtain an accurate estimate of the population response when optimising the feeding program for different objective functions, (c) the effect of changing the value of genetic parameters such as mature protein weight, rate of maturing, feathering rate and the maximum lipid:protein ratio in the gain on the optimum amino acid contents and nutrient densities of broiler feeds, and (d) the effect of variation in nutrient composition of different batches of feed, which have the same nutrient profile but different qualities of the main protein source, on broiler performance. A review of sources of variation in the nutrient content of poultry feed was conducted, and simulation exercises were carried out to determine to what extent broiler performance is affected by the segregation or breakage of pellets into small pieces at the time of delivery and along the feed conveyor within the broiler house, by the change in nutrient quality that might occur along the conveyor, and by the microclimates that develop in a longitudinally ventilated broiler house. The tendency in broiler marketing in most parts of the world is to sell broilers cut up, as portions or deboned after evisceration, rather than selling whole birds. Estimation of the growth rates of carcass parts is therefore of considerable importance if simulation models are to be useful in optimising the feeds and feeding programmes of broilers under different conditions. Allometric equations are used in the EFG broiler growth model to predict the weights of these carcass parts from the weight of body protein at the time. These equations are based on data collected many years ago, and it would be useful to determine whether they are still relevant in the face of announcements by the major broiler breeding companies that tremendous strides have been made in improving breast meat yield, for example, by judicious selection. For the purpose of this investigation it was important to determine to what extent the weights of the physical parts varied at the same body protein weight, thereby enabling a more accurate estimation of the variation that could be expected in these weights when developing a population response model. Towards this end, experiments were conducted to determine the effect of dietary protein content on the performance of Cobb and Ross broilers, including mortality and uniformity, and on the allometric relationships between the physical and chemical components of the body and body protein. The overall objective of these exercises was to address issues relating to the use of simulation models in predicting food intake and growth of broilers, in optimising the amino acid contents and nutrient densities of feeds for broilers, and in representing a population of broilers when the performance of only one bird is simulated at a time.
A study of the Culcoides (Diptera: ceratopogonidae) vectors of African horse sickness to enhance current practical control measures and research methods.
(2011-01-17) Jenkins, Alexander Byron.
African horse sickness virus causes a non-contagious, infectious disease of equids. It is epizootic to sub-Saharan Africa and parts of the Middle East. The epizootics caused by the virus have caused widespread devastation amongst equids worldwide. Fortunately no epizootic has lasted more than 5 years outside of sub- Saharan Africa. It is vectored by species of Culicoides midges (Diptera: Ceratopogonidae) and most importantly by the two Avarita species of C. imicola Keiffer and C. bolitinos Meiswinkel. The literature pertaining to the study and research of the virus, the disease and the vectors is reviewed. Models allowing prediction of future possible outbreaks as well as details of control strategies and findings of researchers are presented and discussed. The virus needs a long term reservoir host in which to overwinter and various theories are discussed. Control measures in South Africa are suggested so that outbreaks of the disease can be reduced.
The use of enzyme supplementation for wheat-barley diets in poultry as a means of improving productive performance.
(2010) Mokoma, Masefo Josephina.; Ciacciariello, Mariana.
The objective of the study was to evaluate the effect of an exogenous multi-blend enzyme ( -glucanase and xylanase) on the performance of the broiler chickens and laying hens fed diets based on wheat and barley. Experiments were conducted on a flock of broilers and two flocks of laying hens. In both cases feed and water were provided ad libitum. The enzyme effect of enzyme addition on the broiler performance involved 2080 day-old male and female chicks in 48 pens, allocated one of four dietary treatments (0, 50, 100 or 200g/ton enzyme supplementation), to 35 days of age. On day 35, ten birds from each treatment were sacrificed for the analysis of the digestive organs weight (gizzards and livers). The trial was divided into two phases: a starter (1 to 21 d) and grower (22 to 35 d). Feed consumption was measured weekly and birds were also weighed weekly. The investigation of enzyme effect in laying hen diets involved 896 birds for each specific period. Each replicate consisted of four cages (four birds per cage) with a common feeder; 16 hens/pen of 56 pens. Eggs were weighed three times a week, feed consumption weekly and birds every weeks. The addition of a multi-blend enzyme significantly improve body weight, body weight gain, food intake, and feed conversion ratio for both sexes (P<0.05) in broiler chickens. There was a significant improvement in egg production in laying hens (P<0.05). Egg weight and egg mass were not significantly improved. Wheat and barley have cell wall components (arabinoxylans and -glucans respectively) which have a negative effect on the nutritive value of these feeds and therefore performance in poultry fed diets based on these ingredients. Addition of an exogenous multi-blend enzyme( -glucanase and xylanase) could help reduce these effects and improve performance and digestibility values in poultry. The null hypothesis was there will be no difference between supplemented and un-supplemented diets based on wheat and barley in performance of poultry. The results of this study suggest that the inclusion of 50 g/ton enzyme helps improve poultry performance, especially in young birds.
The photoperiodic response of male broiler breeders.
(2010) Tyler, Nicola Claire.; Gous, Robert Mervyn.
The expression of photorefractoriness, a feature of seasonal breeding in birds, is important in production species, where egg production and fertility are affected by the photoperiod. Years of selection in meat-type birds have reduced the potential reproductive function, and the environmental manipulation of such genotypes is essential to maximise productivity. While it is known that egg-type pullets no longer exhibit photorefractoriness, and that female broiler breeders do, there is not much information about the response of male broiler breeders to photoperiod. Such information is important when designing lighting programmes for breeding stock, and this study aimed to gain more insight into the male response to photoperiod. A series of experiments was designed in order to achieve this. In the first the response of male broiler breeders to rearing on constant photoperiods was measured, and in the second the effects of age at photostimulation on age at sexual maturity were assessed. The response to an increase in photoperiod during the production cycle was investigated in the third trial. Evidence of photorefractoriness in males was observed, but this was not the case in all birds, possibly due to high variation in some of the fertility traits measured. It seems possible that males do not respond to photoperiod to the same extent as females. Unexpected observations of a response in some birds to early photostimulation, and the high variation observed prompted another experiment in which the heritability of the response to early photostimulation was measured as a potentially revolutionary method of selection, whilst checking that this response is not negatively linked to broiler growth rates. Left and right testis weight data, collected in the above experiments, were analysed for asymmetry. There was no consistent response in testes asymmetry to photoperiod, or evidence that asymmetry in testis weights is as a result of the imposition of genetic or environmental stress. The strong correlation found between testes dimensions and weight suggests that predictions of testis weights could be made through ultrasonics or laparoscopy.
The effect of dietary crude protein, organic selenium and vitamin E on fertility and semen quality of broiler breeder males.
(2008) Bekker, Hester Aletta.; Tyler, Nicola Claire.; Gous, Robert Mervyn.
There are negative influences of selection for broiler growth on the reproductive ability of broiler breeder parents. This is mostly due to problems related to excessive body weight, such as an inability to achieve successful cloacal contact during natural mating. There is also an age-related decline in fertility of broiler breeders. In attempts to prolong the fertile period of the breeders, various forms of management techniques have been employed. These include tools such as feed restriction, photoperiod management, spiking, and possibly even using artificial insemination. The first objective of this thesis was to investigate the possible benefits of feeding broiler breeder males diets containing lower crude protein levels, than given to the females, as a means of possibly tempering growth rate or improving semen quality and fertility. There was a definite improvement in the ability of the spermatozoa of the males on a lower crude protein diet to survive in the female reproductive tract. Males that received higher levels of crude protein were at a disadvantage in that fewer males yielded semen in response to abdominal massage. The second objective of this thesis was to assess the possible benefits in semen quality and fertility, when supplementing the male diets with additional vitamin E or Se in the form of Sel-Plex®. No significant effect of treatment was observed on egg fertility or semen quality.
Predicting the weights of the physical parts of broilers.
(2009) Danisman, Raife.; Gous, Robert Mervyn.
Breeding companies advertise their chickens as having been selected for heavier breast meat. However, when comparisons are made between strains, these are normally made at a common age, and under these conditions the heaviest birds will have the heaviest breast meat yield. More meaningful comparisons would be made by relating breast weight to body protein weight, as these are allometrically related. Two experiments were conducted to test the hypothesis that the allometric relationship for each body part is the same irrespective of strain, sex and feed protein content, i.e. that geneticists have not been successful in changing the allometric relationship between breast meat weight and body protein weight. In the first trial, three strains, two sexes and four feed protein levels were used to 6 weeks of age, and in the second, four strains, two sexes and three feed protein levels were used to 12 weeks. Birds were sampled weekly, and the weights of breast meat (no skin or bone) and the meat and skin of the thigh, drum and wing were recorded before determining the body protein content of each of 1526 broilers. The hypothesis could not be corroborated when the data from the two trials were combined so a further trial was conducted to determine the amount of lipid that is deposited in the meat and skin of each of the commercially important parts of the broiler, on the assumption that differences in lipid deposition between strains, sexes and feed protein levels in the various physical parts would assist in explaining the anomalies in the analyses. It was confirmed in the third trial that the small differences between the observed and predicted weights of the physical parts may be accounted for through varying amounts of lipid deposition in these parts, depending on strain, sex and feed protein level, which must be accounted for when using allometry to predict the weights of the physical parts of the broiler at different stages of growth. The data collected in this series of trials may be used to predict the weights of these physical parts more accurately than has been the case to date.
A dynamic mechanistic anaylsis of the thermal interaction between a broiler chicken and its surrounding environment.
(2010) Blanco, Oscar Adrian.
Chickens, being open thermodynamic systems, maintain a constant exchange of energy and matter with their surrounding environment. In order to avoid reaching thermodynamic equilibrium with the environment the bird makes use of homeostatic mechanisms. These ensure the reduction of the entropy of the system to values that guarantee its integrality. The thermoregulatory response is a major component of the homeostatic machinery of living systems. This induces modifications of physiological parameters of the bird, taking the system “bird” to a new steady state. The achievement of this new state is possible only if the thermoregulatory mechanisms of the birds are able to counteract the environmental demand/burden. A successful thermoregulatory response depends not only on the achievement of that steady state, but also on the compatibility of the value of those parameters with life (especially regarding the value achieved by body temperature) as well as on the time of exposure to the environmental perturbation. Based on those premises, this thesis presents a mechanistic analysis of the thermal interaction between a broiler and its surroundings. The first section of the document introduces the reader to the general concepts of thermodynamics of living systems and physics of heat exchange. The second use mechanistic simulation techniques to represent the environment, the thermal and thermoregulatory properties of a broiler chicken and the interaction between bird and environment. Finally, the third section describes a conceptual simulation model able to predict, over a given period of time, the response of a bird to environmental conditions above those associated with least thermoregulatory effort. Various simulation exercises are reported, the objectives being to study the behaviour of certain variables and to question the validity of current theories of thermoregulation in environmental physiology.
Photic involvement in the reproductive physiology of female domestic fowl.
(2008) Lewis, Peter David.; Gous, Robert Mervyn.
No abstract available.
Modelling nutrient responses and performance of broiler breeders after sexual maturity.
(2007) Nonis, Magalie Kathy.; Gous, Robert Mervyn.
With the worldwide increase in consumption of poultry meat in recent years, the production of hatchable eggs from broiler breeding stock has become a critically important component of the poultry industry. Surprisingly, a perusal of the literature pertaining to broiler breeder nutrition leads to the conclusion that research nutritionists have neglected these birds. It has been assumed in many cases that the research on laying hens is applicable to broiler breeders. However, fundamental differences are apparent between the two strains that should be investigated more comprehensively if the potential of broiler breeder hens is to be achieved. Commercial laying hens have been selected predominantly for increased egg production whereas broilers have been selected for early rapid growth rate. By selecting for improved growth rate, both food consumption and mature weight of these birds has increased (Reddy, 1996), but because of the negative genetic correlation between body weight and egg production (Robinson et al, 1993) reproductive performance has not been improved. Broiler breeder hens differ from commercial laying hens, by their non-normal frequency distribution of egg outputs, their considerable lipid reserves, and by the fact that many do not lay in closed cycle. The practice of restricting feed intake during both the rearing and laying periods has become a standard management procedure in commercial broiler breeder operations and this differs from the manner in which commercial hens are fed. This raises important issues regarding the requirements of these birds for energy, amino acids and other essential nutrients, as the birds do not have the opportunity of meeting their nutrient requirements by adjusting food intake upwards when one or more of these nutrients is deficient in the feed. It is the duty of the nutritionist to provide the correct daily allowance of each nutrient in order to achieve maximum egg output by the flock, but given the variation between hens within a flock, such decisions need to be made on both biological and economic grounds. Improved strains are continually being produced by breeder companies, which exhibit better growth, feed efficiency and productivity. The way in which broiler breeder hens were fed in the past might not be the most effective way to feed the latest strains. Getting the right amount of feed with the right nutrient levels at the right time is the most important part of feeding broiler breeders, and to succeed their daily nutrient requirements need to be known. Information concerning the nutritional requirements of broiler breeder hens is limited in comparison to other types of domesticated poultry. However, enough information is available concerning energy and amino acid nutrition of this type of poultry to enable one to develop models useful for constructing accurate feeding programmes. The most appropriate way of estimating the nutrient requirement of broiler breeder hens during the laying period, or of optimising a feeding strategy, is by the use of simulation models. Emmans and Fisher (1986) suggested that a better approach to the problem of describing requirements and of expressing them quantitatively can be achieved by considering: firstly, the bird’s characteristics, secondly by defining resource scales carefully and thirdly by considering the quantities of each resource needed per unit of function. This approach has a greater chance of success than attempting to measure requirements by direct experimentation. Energy and amino acids are required for growth of tissues, egg production, maintaining normal body temperature, vital life functions and activity. For development of feeding programmes, we are most concerned with the three primary components, maintenance, growth and egg output. There are a number of factors that impact on the total nutrient requirement of the breeder. The maintenance component is affected by body size, environmental temperature, level of activity (housed in floor pens vs. cages) and possibly breed. Regarding the growth component, in the case of broiler breeders during lay the composition of growth needs to be addressed: whether this is only lipid gain or also includes protein gain. Lastly, the egg component is influenced by egg mass and hen age. In order to calculate energy and amino acid requirements, one must have knowledge of the requirements per unit of body protein weight, growth rate and egg mass. By continually monitoring the environmental conditions in the broiler breeder house, as well as body weight, egg weight and egg number, it is possible to estimate the state of the hens at any time and hence the optimum nutrient concentrations that should be fed the next day of the laying period by using the Breeder Model presented in this thesis. Optimising the feeding of broiler breeders during the laying period is made difficult because of the many interacting factors influencing their performance All the hens are not the same, they are not housed in the same environments, and the costs of feeding and the revenue derived from the sale of the product differs from one locality to another. The solution to this problem lies in the use of simulation models to describe the causal relationship between inputs and the predicted responses. This thesis explored new concepts and components for a simulation model to predict the nutrient requirement and performance of broiler breeders after sexual maturity.
Choice feeding as a method of meeting the changing protein requirements of broilers during their growing period.
(2005) Abdella, Mohamed Salih.; Gous, Robert Mervyn.
Broiler production is an important animal production enterprise with potential to make high returns. Increasing feed efficiency and early body weight gain has always been a top priority in the broiler industry. The general objective of broiler nutrition is to maximise production performance and profitability . Nutrition is of major importance in raising chicken, and feed is a major input in poultry production systems, accounting for over 60% of total production costs in commercial poultry sector Renkema (1992). The cost of feed is therefore often a constraint especially in developing countries. For instance, Onyenokwe (1994) observed that high cost of feed ingredients in many African countries has caused many poultry farmers to abandon the industry. The continued rise in feed prices is due to competition for some of the ingredients with human e.g. sorghum, wheat and maize. Broiler farmers are therefore forced to use combinations of feed ingredients of low cost to obtain savings and avoid any further loss of profits. It is therefore important to give special attention to feed and feeding since the rate of feed consumption increases rapidly with advancing age of the birds and good nutrition is reflected in the bird's performance and its products. The profitability of a broiler enterprise depends on the efficient conversion of feed to meat. Broilers have the ability to convert the feeds into meat with a high efficiency. For instance Morris and Njuru (1990) reported that broilers have much higher daily rates of protein deposition than layer chicken strains. This implies that fast-growing strains would require greater daily protein intakes than slow-growing ones. In the past, the major criteria for assessing the performance of broilers has been growth rate and feed conversion ratio (FeR). Diet specifications and feeding programmes have been aimed at maximising these two parameters whereby overall flock performance is calculated based on the total weight of chicken produced from total feed deliveries. With the new developments in understanding of nutritional factors affecting broiler growth and carcass composition, it is now possible to apply sophisticated and yet efficient approaches to feeding broilers.
Meeting the amino acid requirements of growing pigs by manipulating the feeding schedule.
(2006) Butt, Tracy Susan.; Gous, Robert Mervyn.
Three experiments were designed to find the most efficient and cost-effective method of meeting the changing amino acid requirements of male and female pigs during the growing period, using the minimum number of feeds. Trial one involved 144, and trial two 192 commercial crossbred (Landrace x Large White) pigs, eight to a pen, with sexes separate. Trial three involved 24 commercial crossbred (Landrace x Large White) pigs and 24 Dalland pigs each penned separately. The amino acid requirements (g/d) for the pigs in all three trials were determined for male and female Landrace x Large White pigs, respectively, using the Pig Growth Model (EFO Software Natal, 1995). Parameter values used for males and females were: growth rate (B, Id) 0.0107 and 0.0120; protein at maturity (Pmat, kg) 39.0 and 28.0; and lipid at maturity (Lpmat, kg) 2.60 and 3.89 respectively. In trials 1 and 2 two iso-energetic (DE 13.8 MJ/kg) basal feeds were formulated; Basal A being a high crude protein (CP) (347g CP/kg) feed designed to meet the amino acid requirements of a male at 20 kg liveweight, while Basal B was a low protein feed (l34g CP/kg) designed to meet the amino acid requirements of a female at 88 kg, thus providing for the most- and the least-demanding pigs on the trial. In the third trial, two iso-energetic (DE 13.8 MJ/kg) basal feeds were again formulated; Basal A being a high crude protein (CP) (347g CP/kg) feed designed to be 20% higher than the amino acid requirements of a male at 20 kg liveweight, while Basal B was a low protein feed (l34g CP/kg) designed to be 20% lower than the amino acid requirements of a female at 88 kg, once again providing for the most- and the least-demanding pigs on the trial. Trials 1 and 2 began when the median weight of pigs in each pen reached 20kg, and were terminated at a pen median of 85 kg liveweight. In the third trial each pig was started on trial when it reached 20kg and was terminated at a weight of 85kg. The first trial involved a phase feeding schedule (20-40, 40-60 and 60-85kg liveweight). The two basal feeds were blended in different proportions to create three feeds per phase: lysine contents in each of the phases in Treatment 1 (TI) were: 11, 8.68 and 7.26g/kg; in T2 they were 9.93, 7.58 and 6.24g/kg; and in T3, 8.85, 6.48 and 5.22g/kg. From the analysis it was established that ADO and time to reach 85kg were the only variables to show significance. ADO exhibited a significant sex x treatment interaction. There were significant differences between treatments for time taken to reach slaughter weight. In addition to a treatment effect there was a sex x treatment interaction for time to slaughter weight. It was expected that males on Tl and females on T3 would exhibit the most efficient performance for their respective sex since these treatments were specifically formulated to meet their requirements. Midway through the trial the pigs contracted enteritis, this affecting the outcome of the trial by inhibiting the potential growth of the pigs. The results of the trial indicated that dietary protein level affected the time taken to reach slaughter weight. This led to the second trial where four treatments were applied. Three of the four treatments followed a fixed feeding schedule, making use of the two basal feeds and a 1: 1 blend of these. The fourth treatment followed a phase feeding schedule, differing between the males (20-65, 65-75 and 75-85 kg liveweight) and the females (20-35, 35-75 and 75-85 kg liveweight). This treatment also made use of the two basal feeds and a 1: 1 blend of these. From the analysis it was found that there were no significant effects of sex and no interactions between feeding treatments and sex; however, ADO, FI, FCE, back fat thickness, time taken to reach 85kg and cost/kg gain were all significantly affected by the feeding treatments. Carcass lean, carcass lipid and total body lipid were also significantly affected by the feeding treatment. This trial was conducted to determine the extent to which differences in growth rate, food intake and carcass lipid could be altered by dietary means. The effect of the level of feed protein was once again shown to be of importance when feeding growing pigs. The third trial was designed to test the efficiency with which two strains make use of the dietary protein supplied. Three treatments were applied: Tl was a choice-feeding treatment in which the pigs were offered the two basal feeds simultaneously. T2 and T3 followed a phase feeding schedule (20-40, 40-60 and 60-85kg liveweight). The two basal feeds were blended in various proportions to create three feeds per phase: the lysine contents in each of the phases in T2 were: 12.2, 10.0 and 7.26g/kg; and in T3 8.1, 6.7 and 5.1g/kg. There were significant sex effects as well as strain x feeding treatment interactions. All variables, ADG, FI, FCE, time taken to reach 85kg and cost/kg gain, with the exception of back fat thickness, showed significance. It was expected that the Dalland strain would perform better than the Cross strain; however, this was not the case, indicating the need for further research into the possibility of feeding according to the genetic makeup of the animal. The importance of meeting the amino acid requirements of the growing pig was evident when summarising the results of the three trials reported here.
Fibrolytic enzyme activity of herbivore microbial ecosystems.
(2006) Fon, Fabian Nde.; Nsahlai, Ignatius Verla.; Dennison, Clive.; Beukes, Mervyn.
The aim of this study was to determine firstly if there exist variations in fibrolysis among herbivore microbial ecosystems and secondly, the effect on fibre hydrolysis of compositing the most active systems with ruminal microbial ecosystem harvested from a Jersey cow. A literature review pointed to the complexity of carbohydrate (fibre) and how the physical and chemical nature of the forage carbohydrate can present barriers that hinder digestion in the rumen, especially its association with hemicelluloses, pectin, lignin and tannins. Fresh rumen fluid was collected from fistulated herbivores (Jersey cow and sheep) and faecal samples from non-fistulated herbivores (buffalo, horse, impala, camel, elephant, llama, sheep, wildebeest and elephant). Crude protein samples were precipitated with 60% ammonium sulfate. Sample activities were monitored and optimised by incubating with carboxymethyl cellulose (CMC) for 2 h at 39°C. The crude protein samples precipitated from the 11 herbivore microbial ecosystems were active. This was confirmed by an increase in enzyme specific activity with a decrease in total crude protein concentration. In vitro pH optimisation showed a broad range of activity for all ecosystems (4.5-8.0) but for the zebra, horse and elephant which peaked at pH 5. In experiment two (Chapter 4), seasonal variation of the enzymes (exocellulase, endocellulase, cellobiase and xylanase) were monitored through winter and summer. Enzyme specific activity of exocellulase, endocellulase, cellobiase and xylanase were determined by incubation with the specific substrates, crystalline cellulose, CMC, pNPG and xylan, respectively. The amount of reducing sugar released was used to determine the enzyme specific activity. Exocellulase analysis was suitable in winter while summer was preferred for carboxymethyl cellulase and xylanase due to their relative abundance. Cellobiase analysis did not depend on any particular season. Eleven herbivore microbial ecosystems were characterised according to their fibrolytic enzyme specific activities. Enzyme catalytic activities were calculated from kinetic parameters (Km and Vmax) obtained from Eisenthal and Cornish-Bowded plots (Chapter 5). Fibrolytic enzyme expression as well as their activities differed among the 11 ecosystems (P
Testing for passive transfer of immunity in foals, and an evaluation of the African horse sickness vaccination schedule.
(2005) Crow, Linnet Jean Isobel.; Young, Marion Belinda.; Bradford, Gail Smith.
This thesis comprises an introductory review of the literature, followed by reports of two experiments which are presented in the form of scientific papers. For this reason, there may be some repetition between chapters, particularly in terms of experimental procedure. To avoid unnecessary repetition, a single list of references is given at the end of the thesis. For the sake of completeness , several appendices are attached to Chapters Two and Three which would not ordinarily be included in a scientific paper. The literature review looks at the passive transfer of immunity from the mare to the foal, the consequences of failure of passive transfer of immunity and different methods of testing whether the transfer of passive immunity has occurred. The review concludes with a discussion of vaccination programmes against African horse sickness. Trial One evaluated different tests for determining whether the transfer of passive immunity from mare to foal has occurred in order to determine which of these tests should be used preferentially. A single radial immunodiffusion test was used as the reference standard. A series of samples .was taken from a group of foals and tested using four methods: single radial immunodiffusion , glutaraldehyde coagulation, zinc sulphate turbidity and protein refractometer tests. Trial Two explored African horse sickness vaccination programmes, focusing on when to vaccinate foals for the first time. A series of samples was taken from a group of foals from birth until two months after their second set of African horse sickness vaccinations (one year old). The samples were tested for the presence of African horse sickness antibodies for each of the nine serotypes to determine when maternal immunity fades and to evaluate the effect of each vaccination on the level of immunity.
The effects of nutritional management on behaviour in thoroughbred racehorses.
(2007) Hackland, Jean.; Young, Marion Belinda.; Gous, Robert Mervyn.
This dissertation is the product of two behaviour studies and an in vitro fermentation trial. Both behaviour studies were conducted at the Ashburton Racehorse-Training Centre in Ashburton, near Pietermaritzburg in KwaZulu- Natal. The first behaviour study evaluated differences in behaviours obtained through feeding either twice or four times daily. This trial showed (P
Constraints on smallholder dairying in Swaziland : Manzini Region & surrounding areas.
(2005) Malima, Boyce Thabo.; Nsahlai, Ignatius Verla.
Summary: Swaziland has long had a disparity between the supply and demand of milk. Even at present milk production continues to be less than the market demand. The quantitative contribution of smallholder dairy farmers to local milk production remains unknown because of poor record keeping. This study was aimed at attaining a clear understanding of the dynamics of smallholder dairying in Swaziland, including the identification and understanding of the constraints faced by farmers in dairying, with the hope of devising workable solutions to them. A sample of 118 smallholder dairy farms were covered in this study, with a total herd of 306 lactating cows, comprising mainly of Jerseys and Holstein Friesians, with some cross breeds. There were no significant differences in mean milk yield/cow with respect to farmer gender (P > 0.05) and Agro-ecological zone location (P > 0.05) of the farms. Milking frequency had a significant effect on milk yield, since cattle milked once a day had lower (P < 0.05) milk yields than those milked twice a day. The cattle had extensively long calving intervals i.e. 448 ± 166 days, ranging from 292 to 1082 days. Low milk yield and poor reproductive performance of cattle were found to be mainly due to poor nutrition, breeding practices and stock quality. These are primarily a result of insufficient farmer training and inadequate technical assistance, scarce availability of quality stock, lack of investment resources and market support that includes favourable milk prices for farmers to make money. This performance of the Swazi smallholder dairy herd was then evaluated by comparing it to the performance of a larger, well-managed herd of known pedigree. Lactation records from 252 Jersey cows and 108 Holstein Friesian cows were obtained from Cedara Agricultural Research Institute, covering the periods; July, 2002 to July, 2004 and November, 2002 to April, 2004, respectively. Cows were grouped by parity and calving season and the gamma function proposed by Wood (Y = An(b) e(-cn)) was used to fit standard lactation curves on group data. The curve parameters A and b increased with parity, while that of c and s (persistency of lactation at peak) decreased, producing standard lactation curves save for the Holstein Friesian summer calvers, which produced atypical curves. The R(2) values (goodness of fit) increased with parity. Animal parity and calving season were found to influence the peak and shape of the lactation curves and their parameter estimates. The performance of the Swazi smallholder herd showed a mean deviation of the observed daily milk yield of the Holstein Friesian breed from the expected yield to be - 3.47 (SD 6.052) kg and that of the Jersey breed was - 16.92 (SD 5.473) kg. The mean proportional deviation of observed milk yield from the expected yield for the Holstein Friesian breed was - 0.3 (SD 0.37) and that of the Jersey breed to be - 0.6 (SD 0.19). The proportional milk yield deviation of the Holstein Friesian breed can be explained using the equation Y = O.1322(SE = 0.1293) x - 2.3581 (SE = 0.20639), where x = expected milk yield and Y is the proportional deviation of the observed milk yield deviation from the expected milk yield. With respect to the smallholder Jersey breed, no relationship was found that could explain the proportional milk yield deviation. The smallholder herd was shown to be underperforming, considering the potential for higher milk yields of the two breeds. In the quest to gain a greater understanding of the dynamics of smallholder dairying, the sample of 118 farmers was further analysed using multivariate statistics to categorise them based on their herd sizes, herd structures, management and success perceptions in dairying. The analysis produced three clusters (categories): cluster 1 had the largest herd sizes and poor milk production efficiency; cluster 2 had intermediate herd sizes, the highest number of farmers and more efficient milk production per cow. This cluster, however, had the highest proportion of calf mortalities. Cluster 3 had the smallest herd size, the lowest calf to cow ratio and the second highest calf mortality. Record keeping across all clusters was very poor and the average milk yield per cow was generally low. Most of the farmers do not appreciate the importance of annual calving of their cows as an integral part of the success of their dairy projects and winter feed supplementation is very poor across all the clusters. There remains a great need for the enlightenment of the farmers on the importance of good nutrition, breeding, calf rearing and record keeping in successful dairying.

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