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dc.creatorAlexandre, Kabamba B.
dc.creatorGray, Elin S.
dc.creatorLambson, Bronwen E.
dc.creatorMoore, Penny L.
dc.creatorChoge, Isaac A.
dc.creatorMlisana, Koleka Patience.
dc.creatorAbdool Karim, Salim Safurdeen.
dc.creatorMcMahon, James.
dc.creatorO'Keefe, Barry.
dc.creatorChikwamba, Rachel.
dc.creatorMorris, Lynn.
dc.date.accessioned2012-11-29T06:19:44Z
dc.date.available2012-11-29T06:19:44Z
dc.date.created2010
dc.date.issued2010
dc.identifier.citationAlexandre, K.B. et al. 2010. Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin. Virology 402 (1), pp. 187–196.en
dc.identifier.issn0042-6822
dc.identifier.urihttp://dx.doi.org/10.1016/j.virol.2010.03.021en
dc.identifier.urihttp://hdl.handle.net/10413/8042
dc.description.abstractGriffithsin (GRFT), Cyanovirin-N (CV-N) and Scytovirin (SVN) are lectins that inhibit HIV-1 infection by binding to multiple mannose-rich glycans on the HIV-1 envelope glycoproteins (Env). Here we show that these lectins neutralize subtype C primary virus isolates in addition to Env-pseudotyped viruses obtained from plasma and cervical vaginal lavages. Among 15 subtype C pseudoviruses, the median IC50 values were 0.4, 1.8 and 20.1 nM for GRFT, CV-N and SVN, respectively, similar to what was found for subtype B and A. Analysis of Env sequences suggested that concomitant lack of glycans at positions 234 and 295 resulted in natural resistance to these compounds, which was confirmed by site-directed mutagenesis. Furthermore, the binding sites for these lectins overlapped that of the 2G12 monoclonal antibody epitope, which is generally absent on subtype C Env. This data support further research on these lectins as potential microbicides in the context of HIV-1 subtype C infection.en
dc.language.isoenen
dc.publisherElsevier.en
dc.subjectHIV (Viruses)en
dc.subject.otherGriffithsin.en
dc.subject.otherCyanovirin-N.en
dc.subject.otherScytovirin.en
dc.subject.otherHIV-1 subtype C.en
dc.subject.otherMicrobicides.en
dc.subject.other2G12 monoclonal antibody.en
dc.titleMannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin.en
dc.typePeer reviewed journal articleen


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