Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin.

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dc.creator Alexandre, Kabamba B.
dc.creator Gray, Elin S.
dc.creator Lambson, Bronwen E.
dc.creator Moore, Penny L.
dc.creator Choge, Isaac A.
dc.creator Mlisana, Koleka.
dc.creator Abdool Karim, Salim Safurdeen.
dc.creator McMahon, James.
dc.creator O'Keefe, Barry.
dc.creator Chikwamba, Rachel.
dc.creator Morris, Lynn.
dc.date.accessioned 2012-11-29T06:19:44Z
dc.date.available 2012-11-29T06:19:44Z
dc.date.created 2010
dc.date.issued 2010
dc.identifier.citation Alexandre, K.B. et al. 2010. Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin. Virology 402 (1), pp. 187–196. en
dc.identifier.issn 0042-6822
dc.identifier.uri http://dx.doi.org/10.1016/j.virol.2010.03.021 en
dc.identifier.uri http://hdl.handle.net/10413/8042
dc.description.abstract Griffithsin (GRFT), Cyanovirin-N (CV-N) and Scytovirin (SVN) are lectins that inhibit HIV-1 infection by binding to multiple mannose-rich glycans on the HIV-1 envelope glycoproteins (Env). Here we show that these lectins neutralize subtype C primary virus isolates in addition to Env-pseudotyped viruses obtained from plasma and cervical vaginal lavages. Among 15 subtype C pseudoviruses, the median IC50 values were 0.4, 1.8 and 20.1 nM for GRFT, CV-N and SVN, respectively, similar to what was found for subtype B and A. Analysis of Env sequences suggested that concomitant lack of glycans at positions 234 and 295 resulted in natural resistance to these compounds, which was confirmed by site-directed mutagenesis. Furthermore, the binding sites for these lectins overlapped that of the 2G12 monoclonal antibody epitope, which is generally absent on subtype C Env. This data support further research on these lectins as potential microbicides in the context of HIV-1 subtype C infection. en
dc.language.iso en en
dc.publisher Elsevier. en
dc.subject HIV (Viruses) en
dc.subject.other Griffithsin. en
dc.subject.other Cyanovirin-N. en
dc.subject.other Scytovirin. en
dc.subject.other HIV-1 subtype C. en
dc.subject.other Microbicides. en
dc.subject.other 2G12 monoclonal antibody. en
dc.title Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin. en
dc.type Peer reviewed journal article en

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