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dc.contributor.advisorParboosing, Raveen.
dc.creatorSingh, Varish.
dc.date.accessioned2010-08-23T09:51:57Z
dc.date.available2010-08-23T09:51:57Z
dc.date.created2009
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/10413/504
dc.descriptionThesis(MMed.)-University of KwaZulu-Natal, 2009.en_US
dc.description.abstractThe development and optimization of anti-viral screening methods are essential to develop newer more effective, treatments against HIV. The XTT method is a widely described method for antiviral screening. Both continuous HIVinfected cells and experimentally infected T-cells have been used in the XTT assay. We compared these methods to screen several plant-derived extracts for cytotoxicity. Several considerations were taken into account when performing these tests (effect of media, solvents and plant enymes). Experiments were performed to investigate these effects. In addition, p24 and viral load quantification were compared as antiviral screening methods. The study showed that several modifications were necessary when performing the XTT assay on plant extracts, due to the effect of media, solvents and plant enymes. The XTT assays and p24 assays performed using experimentally infected cells are far more specific than those using chronically infected cells. The use of viral loads as an antiviral screening method consistently demonstrated the expected efficacy of AZT.en_US
dc.language.isoen_ZAen_US
dc.subjectMedical virology.en_US
dc.subjectHIV infections--Molecular aspects.en_US
dc.subjectHIV (Viruses)--Identification.en_US
dc.subjectHIV (Viruses)--Molecular aspects.en_US
dc.subjectTheses--Virology.en_US
dc.titleThe development, optimisation and comparison of various virological assays and their uses in antiviral assessment of compounds wih potential anti-HIV activity.en_US
dc.typeThesisen_US


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