The development, optimisation and comparison of various virological assays and their uses in antiviral assessment of compounds wih potential anti-HIV activity.

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dc.contributor.advisor Parboosing, Raveen.
dc.creator Singh, Varish.
dc.date.accessioned 2010-08-23T09:51:57Z
dc.date.available 2010-08-23T09:51:57Z
dc.date.created 2009
dc.date.issued 2009
dc.identifier.uri http://hdl.handle.net/10413/504
dc.description Thesis(MMed.)-University of KwaZulu-Natal, 2009. en_US
dc.description.abstract The development and optimization of anti-viral screening methods are essential to develop newer more effective, treatments against HIV. The XTT method is a widely described method for antiviral screening. Both continuous HIVinfected cells and experimentally infected T-cells have been used in the XTT assay. We compared these methods to screen several plant-derived extracts for cytotoxicity. Several considerations were taken into account when performing these tests (effect of media, solvents and plant enymes). Experiments were performed to investigate these effects. In addition, p24 and viral load quantification were compared as antiviral screening methods. The study showed that several modifications were necessary when performing the XTT assay on plant extracts, due to the effect of media, solvents and plant enymes. The XTT assays and p24 assays performed using experimentally infected cells are far more specific than those using chronically infected cells. The use of viral loads as an antiviral screening method consistently demonstrated the expected efficacy of AZT. en_US
dc.language.iso en_ZA en_US
dc.subject Medical virology. en_US
dc.subject HIV infections--Molecular aspects. en_US
dc.subject HIV (Viruses)--Identification. en_US
dc.subject HIV (Viruses)--Molecular aspects. en_US
dc.subject Theses--Virology. en_US
dc.title The development, optimisation and comparison of various virological assays and their uses in antiviral assessment of compounds wih potential anti-HIV activity. en_US
dc.type Thesis en_US

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