Isolation of entomopathogenic gram positive spore forming bacteria effective against coleoptera.

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dc.contributor.author Du Rand, Nicolette.
dc.creator Du Rand, Nicolette.
dc.date.accessioned 2010-09-20T10:58:23Z
dc.date.available 2010-09-20T10:58:23Z
dc.date.created 2009
dc.date.issued 2009
dc.identifier.uri http://hdl.handle.net/10413/1235
dc.description Thesis (Ph.D.) - University of KwaZulu-Natal, Pietermaritzburg, 2009. en_US
dc.description.abstract Fourteen spore-forming bacterial strains were isolated and screened for entomopathogenic activity. Five displayed toxicity towards the common mealworm, Tenebrio molitor L., (Coleoptera: Tenebrionidae). The majority of the isolates were obtained from insect larvae and insect rich environments. The three bacterial species identified were Bacillus thuringiensis Berliner, Brevibacillus laterosporus Laubach and Bacillus cereus Frankland and Frankland. Bioassays were conducted using T. molitor larvae. The one isolate of B. cereus required the highest concentration of bacterial cells to achieve its LC50, whereas one of the isolates of B. laterosporus required the lowest cell concentration to achieve its LC50. Dose response curves were generated for the five best isolates, which showed that the isolate of B. laterosporus (NDR2) was substantially more toxic than the other isolates.
dc.language.iso en_ZA en_US
dc.subject Bacillus (Bacteria) en_US
dc.subject Bacillus thuringiensis. en_US
dc.subject Bacillus cereus. en_US
dc.subject Microbial insecticides. en_US
dc.subject Insect pests--Biological control. en_US
dc.subject Biological pest control agents. en_US
dc.subject Sugarcane--Diseases and pests--South Africa. en_US
dc.subject Tenebrionidae--South Africa. en_US
dc.subject Scarabaeidae--South Africa. en_US
dc.subject Theses--Plant pathology. en_US
dc.title Isolation of entomopathogenic gram positive spore forming bacteria effective against coleoptera. en_US
dc.type Thesis en_US

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