Micropropagation of tulbaghia species.
Ngunge, Viwe Nomzamo Precious.
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Tulbaghia (Alliaceae) is a genus of plants with medicinal, ornamental and nutritive value. Different plant parts such as roots, bulbs, leaves and flowers are used in the treatment of a variety of conditions. The bulbs of Tulbaghia violacea are used as a remedy for pulmonary tuberculosis as well as an anthelmintic. Due to the extensive harvesting of plants in this genus, the genus is susceptible to overexploitation and may eventually become threatened with extinction. It was therefore the aim of this study to systematically examine the micropropagation of Tulbaghia ludwigiana and Tulbaghia violacea, as well as to evaluate the antimicrobial and phytochemical properties of micropropagated plants. Seeds of T. ludwigiana and T. violacea were successfully decontaminated using 70% ethanol, 1% Benlate and 3.5% NaOCl. Temperature played a significant role in the germination of both species while light did not play a significant role in this process. Light did not play a significant role in the stomatal density of T. violacea seedlings. Hypocotyls were the regenerative part of the seedlings in both species. A low number of shoots was yielded by the combination of various concentrations of NAA and mTR in the growth medium in both species. There were more isoprenoid cytokinins than there were aromatic cytokinins in each of the seedling sections of T. violacea that were analysed, with tZ being the predominant isoprenoid cytokinin, while BA was the predominant aromatic cytokinin. Shoots of both species were successfully rooted in a medium with IBA, while some shoots had simultaneously rooted during shoot multiplication. Potting soil and vermiculite were used in the acclimatization of both species, where T. violacea plantlets acclimatized successfully. This was not the case for T. ludwigiana. Micropropagated T. violacea plantlets contained higher concentrations of phytochemical compounds and displayed better antibacterial activity than outdoor-grown plants.
- Masters Degrees (Botany)