Show simple item record

dc.contributor.advisorMasola, Bubuya.
dc.creatorLanga, Silvana Paula Octavio.
dc.date.accessioned2019-01-21T09:11:03Z
dc.date.available2019-01-21T09:11:03Z
dc.date.created2016
dc.date.issued2016
dc.identifier.urihttp://hdl.handle.net/10413/16010
dc.descriptionMaster of Science in Biochemistry. University of KwaZulu-Natal, Durban 2016.en_US
dc.description.abstractDiabetes prevalence increases yearly with an expected increase to 642 million people by the year 2040. Conventional treatment of diabetes involves insulin therapy and other synthetic drugs. However, drawbacks of these forms of treatments include high cost and adverse side effects. There is therefore, a renewed focus on the use of plants and plant derived products for treatment due to their safety and limited side effects. Oleanolic acid (OA) is a plant derived triterpenoid compound that has been shown to suppress expression of genes encoding hepatic gluconeogenic enzymes and in so doing inhibits hepatic glucose production. Centella asiatica (L.) Urban (CA), an herbaceous plant belonging to the family Umbelliferae, has been traditionally used for the management of diabetes. Thus, this study was aimed at investigating the effects of OA and CA on selected enzymes of carbohydrate metabolism in muscle and skin of streptozotocin- or 10% fructose-streptozotocin-induced diabetic Sprague-Dawley rats. Non-diabetic and diabetic rats were treated with OA, 500 mg/kg of CA, 1000 mg/kg of CA and metformin over 14 days. OA increased HK and PFK activity of diabetic animals by 82% and 432% (p < 0.05) in muscle and 17.7% and 71% in skin respectively. OA also lowered FBPase activity by 24%. Similarly, 500 mg/kg of CA increased HK and PFK activity of diabetic animals by 4.4% and 624% (p < 0.05) in muscle and 11% and 35% in skin respectively. OA and CA reduced PFK expression in both muscle and skin of diabetic animals. CA also increased GS activity by 346% (p < 0.05) in skin of diabetic animals. Treatment with CA decreased ALT and AST levels and reduced damage to muscle fibre and skin epidermis of diabetic animals in histological studies. The findings of this study indicate that hypoglycaemic effect OA and CA may occur through modulation of glycolysis, gluconeogenesis and glycogen synthesis. Tissue damage and structural changes in muscle and skin caused by diabetes may be reduced by treatment with CA.en_US
dc.language.isoen_ZAen_US
dc.subject.otherCentella asiatica.en_US
dc.subject.otherType-11 diabetes.en_US
dc.subject.otherOleanolic acid.en_US
dc.subject.otherRat muscle.en_US
dc.subject.otherRat skin.en_US
dc.titleEffects of oleanolic acid and Centella asiatica on enzymes of carbohydrate metabolism in muscle and skin of streptozotocin-induced diabetic Sprague-Dawley rats.en_US
dc.typeThesisen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record